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Catalog No: OKCD07641
Size:96WELLS
Price: $750.00
SKU
OKCD07641
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Datasheets/ManualsPrintable datasheet for REG1A ELISA Kit (Human) (OKCD07641)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Regenerating Islet Derived Protein 1 Alpha (REG1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Regenerating Islet Derived Protein 1 Alpha (REG1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Regenerating Islet Derived Protein 1 Alpha (REG1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Regenerating Islet Derived Protein 1 Alpha (REG1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Regenerating Islet Derived Protein 1 Alpha (REG1a) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Regenerating Islet Derived Protein 1 Alpha (REG1a) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
REG1A Microplate96 Wells (12 x 8 Well strips)
REG1A Lyophilized Standard2
100X REG1A HRP-Detector Anitbody1 x 120 ÂuL
100X Avidin-HRP Conjugate1 x 120 ÂuL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 6.7 pg/mL
Gene SymbolREG1A
Gene Full Nameregenerating family member 1 alpha
Alias SymbolsICRF;islet cells regeneration factor;islet of langerhans regenerating protein;lithostathine-1-alpha;P19;Pancreatic stone protein;pancreatic stone protein, secretory;pancreatic thread protein;protein-X;PSP;PSPS;PSPS1;PTP;REG;REG-1-alpha;regenerating islet-derived protein 1-alpha;regenerating protein I alpha.
NCBI Gene Id5967
Protein NameLithostathine-1-alpha
Description of TargetMight act as an inhibitor of spontaneous calcium carbonate precipitation. May be associated with neuronal sprouting in brain, and with brain and pancreas regeneration.
Uniprot IDP05451
Protein Accession #NP_002900.2
Nucleotide Accession #NM_002909.4
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