Active Recombinant SARS-CoV-2 Spike S1 Protein (OPAN06663)

Catalog No: OPAN06663

Size:100UG

Measured by its binding ability in a functional ELISA. Immobilized nCoV-S1 at 2 g/mL (100 L/well) can bind Recombinant Human ACE2 with a linear range of 0.06-0.3ug/mL.

Immobilized Recombinant Human ACE2 at 2ug/mL (100 uL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL.

Recombinant 2019-nCoV Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.

Product Info

• Tested Species Reactivity: 2019-nCoV
• Predicted Species Reactivity: SARS-CoV-2 / COVID-19 Virus, Severe acute respiratory syndrome coronavirus 2
• Product Format: Lyophilized PBS (pH 7.4)
• Application: SDS-PAGE
  Endotoxin Level: < 0.1 EU/ug of the protein by LAL method.
• Reconstitution and Storage: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortexing or vigorously pipetting the protein. For long-terms storage, it is recommended to add a carrier protein or stabilizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose) and aliquot the reconstituted protein solution to minimize freeze/thaw cycles.
  Store the lyophilized protein at -20°C to -80°C long-term. After reconstitution, the product is stable at -20°C for 3 months, at 2-8°C for up to 1 week. This product is stable at ≤ -70°C for up to 6 months from date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze/thaw cycles.
• Purity: >90% by SDS-PAGE.
• Biological Activity: 1) Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2 ug/mL (100 uL/well) can bind Recombinant SARS-CoV-2 Spike S1, the EC₅₀ of SARS-CoV-2 Spike S1 is 6.79 ng/ml.
  2) Immobilized Human ACE2 on COOH Chip can bind SARS-CoV-2 Spike S1 with an affinity constant of 90.8 nM as determined in a SPR assay.
• Protein Sequence: Val11-Arg682 of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with an Fc, 6 x His tag at the C-terminus.
• Source: HEK293 Cells
• Tag: Fc, 6 x His tag at the C-terminus

Target Info

• Gene Symbol: S
• Gene Full Name: surface glycoprotein
• Alias Symbols: GU280_gp02;spike glycoprotein;surface glycoprotein.
• NCBI Gene Id: 43740568
• Protein Name: surface glycoprotein
• Description of Target: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
• Protein Accession #: YP_009724390.1
• Protein Size (# AA): Active|Recombinant