Recombinant SARS-CoV-2 Spike S1 Protein (OPAN06663)

Catalog No: OPAN06663

Size:100 ug

Measured by its binding ability in a functional ELISA. Immobilized nCoV-S1 at 2 g/mL (100 L/well) can bind Recombinant Human ACE2 with a linear range of 0.06-0.3ug/mL.

Immobilized Recombinant Human ACE2 at 2ug/mL (100 uL/well) can bind Recombinant 2019-nCoV Spike S1-TEVS-hFc-His with a linear range of 78-82.5 ng/mL.

Recombinant 2019-nCoV Spike S1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 130-160 kDa.

Product Info

• Tested Species Reactivity: 2019-nCoV
• Predicted Species Reactivity: 2019-nCoV
• Product Format: Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4
• Application: SDS-PAGE
• Additional Information: Description: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
  Endotoxin Level: < 0.1 EU/ug of the protein by LAL method.
• Reconstitution and Storage: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Store the lyophilized protein at -20C to -80C for long term. After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week. Avoid repeated freeze/thaw cycles.
• Purity: >95% by SDS-PAGE.
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized nCoV-S1 at 2 g/mL (100 L/well) can bind Recombinant Human ACE2 with a linear range of 0.06-0.3ug/mL.
• Protein Sequence: Val11-Arg682 of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with an Fc, 6 x His tag at the C-terminus.
• Source: HEK293 cells
• Tag: Fc, 6 x His tag at the C-terminus

Target Info

• Gene Symbol: S
• Alias Symbols: spike glycoprotein
• NCBI Gene Id: 43740568
• Protein Name: surface glycoprotein
• Protein Accession #: YP_009724390.1