Catalog No: OPAN06663
Size:100 ug
Price: $349.00
SKU
OPAN06663
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/Manuals | Printable datasheet for OPAN06663 |
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Tested Species Reactivity | 2019-nCoV |
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Predicted Species Reactivity | 2019-nCoV |
Product Format | Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4 |
Application | SDS-PAGE |
Additional Information | Description: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity. |
:: | Endotoxin Level: < 0.1 EU/ug of the protein by LAL method. |
Reconstitution and Storage | Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Store the lyophilized protein at -20C to -80C for long term. After reconstitution, the protein solution is stable at -20 C for 3 months, at 2-8 C for up to 1 week. Avoid repeated freeze/thaw cycles. |
Purity | >95% by SDS-PAGE. |
Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized nCoV-S1 at 2 g/mL (100 L/well) can bind Recombinant Human ACE2 with a linear range of 0.06-0.3ug/mL. |
Protein Sequence | Val11-Arg682 of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with an Fc, 6 x His tag at the C-terminus. |
Source | HEK293 cells |
Tag | Fc, 6 x His tag at the C-terminus |
Gene Symbol | S |
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Alias Symbols | spike glycoprotein |
NCBI Gene Id | 43740568 |
Protein Name | surface glycoprotein |
Protein Accession # | YP_009724390.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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