Catalog No: OPAN06662
Size:100 ug
Price: $349.00
SKU
OPAN06662
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for OPAN06662
Product Info
Tested Species Reactivity2019-nCoV
Predicted Species Reactivity2019-nCoV
Product FormatLyophilized from a 0.22 um filtered solution of PBS, pH 7.4
ApplicationSDS-PAGE
::Endotoxin Level: < 0.1 EU/ug of the protein by LAL method.
Reconstitution and StorageReconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. For optimal storage, aliqout into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze/thaw cycles.
Purity>95% by SDS-PAGE; > 95% by HPLC
Biological ActivityMeasured by its binding ability in a functional ELISA. Immobilized Recombinant 2019-nCoV RBD-6His at 2 ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.53 ng/mL. Immobolized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay.
Protein SequenceArg319-Phe541 Arg319-Phe541 of 2019-ncov Spike RBD-His (Accession #YP_009724390.1) fused with a 6 x His tag at the C-terminus.
SourceHEK293 cells
TagC-terminal 6xHis-tag
Gene SymbolS
Alias Symbolsspike glycoprotein
NCBI Gene Id43740568
Protein Namesurface glycoprotein
Protein Accession #YP_009724390.1
Molecular Weight26 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express S.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express S.
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