Recombinant SARS-CoV-2 Spike RBD Protein (OPAN06662)

Catalog No: OPAN06662

Size:100 ug

Immobilized Recombinant 2019-nCoV RBD-6His at 2ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 8-80 ng/mL.

Recombinant 2019-nCoV Spike RBD Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 36 kDa.

Product Info

• Tested Species Reactivity: 2019-nCoV
• Predicted Species Reactivity: 2019-nCoV
• Product Format: Lyophilized from a 0.22 um filtered solution of PBS, pH 7.4
• Application: SDS-PAGE
  Endotoxin Level: < 0.1 EU/ug of the protein by LAL method.
• Reconstitution and Storage: Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week. For optimal storage, aliqout into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze/thaw cycles.
• Purity: >95% by SDS-PAGE; > 95% by HPLC
• Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant 2019-nCoV RBD-6His at 2 ug/mL (100 uL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.53 ng/mL. Immobolized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay.
• Protein Sequence: Arg319-Phe541 Arg319-Phe541 of 2019-ncov Spike RBD-His (Accession #YP_009724390.1) fused with a 6 x His tag at the C-terminus.
• Source: HEK293 cells
• Tag: C-terminal 6xHis-tag

Target Info

• Gene Symbol: S
• Alias Symbols: spike glycoprotein
• NCBI Gene Id: 43740568
• Protein Name: surface glycoprotein
• Protein Accession #: YP_009724390.1
• Molecular Weight: 26 kDa