Catalog No: OPCA335983
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OPCA335983
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Recombinant SARS-CoV-2 Spike Glycoprotein (OPCA335983)

Datasheets/ManualsPrintable datasheet for OPCA335983
Product Info
Predicted Species ReactivitySARS-CoV-2
Product FormatLyophilized powder
Additional InformationBiological Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 ug/ml can bind Biotinylated Anti-SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 106.2-131.2 ng/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 ug/ml can bind SARS-CoV-2-S1-RBD, the EC50 of SARS-CoV-2-S1-RBD protein is 8.363-12.82 ng/ml.
::Biological Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 ug/ml can bind Biotinylated Anti-SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 106.2-131.2 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 ug/ml can bind SARS-CoV-2-S1-RBD, the EC50 of SARS-CoV-2-S1-RBD protein is 8.363-12.82 ng/ml.
Reconstitution and StorageWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
PurityGreater than 90% as determined by SDS-PAGE.
Biological Activity1. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S1-RBD at 2 ug/ml can bind Biotinylated Anti-SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S1-RBD protein is 106.2-131.2 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized human ACE2 at 2 ug/ml can bind SARS-CoV-2-S1-RBD, the EC50 of SARS-CoV-2-S1-RBD protein is 8.363-12.82 ng/ml.
Protein SequenceRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF
Storage BufferLyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
SourceMammalian cell
Protein Range319-541 aa
TagC-terminal 6xHis-mFc-tagged
Gene SymbolS
Protein NameSpike glycoprotein
Swissprot IdP0DTC2
Protein Size (# AA)319-541 aa
Molecular Weight51.1 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express S,S1-RBD.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express S,S1-RBD.
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