Catalog No: OPCA335982
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OPCA335982
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Recombinant SARS-CoV-2 Spike Glycoprotein (OPCA335982)

Datasheets/ManualsPrintable datasheet for OPCA335982
Product Info
Predicted Species ReactivitySARS-CoV-2
Product FormatLyophilized powder
ApplicationSDS-PAGE
Additional InformationBiological Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S at 2 ug/ml can bind human ACE2 , the EC50 of SARS-CoV-2-S protein is 56.64 - 103.6 ng/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S at 2 ug/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S protein is 36.79-48.87 ng/ml.
::Biological Activity: 1. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S at 2 ug/ml can bind human ACE2 , the EC50 of SARS-CoV-2-S protein is 56.64 - 103.6 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S at 2 ug/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S protein is 36.79-48.87 ng/ml.
Reconstitution and StorageWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
PurityGreater than 85% as determined by SDS-PAGE.
Biological Activity1. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S at 2 ug/ml can bind human ACE2 , the EC50 of SARS-CoV-2-S protein is 56.64 - 103.6 ng/ml. 2. Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-S at 2 ug/ml can bind SARS-CoV-2-S Antibody , the EC50 of SARS-CoV-2-S protein is 36.79-48.87 ng/ml.
Protein SequenceVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRAR
Storage BufferLyophilized from a 0.2 um filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0
SourceMammalian cell
Protein Range16-685 aa
TagN-terminal 10xHis-tagged and C-terminal Flag-tagged
Gene SymbolS
Protein NameSpike glycoprotein
Swissprot IdP0DTC2
Protein Size (# AA)16-685 aa
Molecular Weight79.6 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express S,S1.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express S,S1.
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