RBX1 Recombinant Protein (Human) (OPED00126)

Catalog No: OPED00126

Size:25UG

Figure: SDS-PAGE image of Rbx1, N-terminally His6 tagged (Coomassie blue stained). Lane 1: MW markers (top to bottom) 200, 116, 97, 66, 55, 45, 36, 29, 24, 20, 14.2, 6.5 kDa. Lane 2: Rbx1 (human) (recombinant) (His)

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Product Format: Liquid. In 50mM TRIS, pH 8.0, containing 50mM NaCl and 1mM DTT.
• Host: E. Coli
• Additional Information: Cullin-based E3 ligases, such as SCF (Skp1-Cdc53-F-box protein), are a large family of multi-subunit ubiquitin ligases with diverse cellular functions, including the regulation of the cell cycle, of the DNA damage response, and of various transcription factors. These ligases are composed of one of six mammalian cullin homologs (Cul1, Cul2, Cul3, Cul4a, Cul4b, and Cul5), the Ring finger containing protein Rbx1/Roc1, and cullin homolog-specific adaptor and substrate recognition subunits. Activation of cullin-based ligases require the covalent modification of a conserved lysine residue in the cullin protein with the ubiquitin-like protein NEDD8. This modification is catalyzed by the Rbx1 subunit.
• Reconstitution and Storage: Store at -80C
• Purification: Purified by nickel sepharose affinity chromatography.
• Purity: >98% (SDS-PAGE)
• Reference: Substrate-mediated regulation of cullin neddylation: E.H. Chew & T. Hagen; J. Biol. Chem. 282, 17032 (2007), Abstract;
  A hitchhiker’s guide to the cullin ubiquitin ligases: A.R. Willems, et al.; Biochim. Biophys. Acta 1695, 133 (2004), Abstract;
  Nedd8 on cullin: building an expressway to protein destruction: Z.Q. Pan, et al.; Oncogene 23, 1985 (2004), Abstract;

Target Info

• Gene Symbol: RBX1
• Gene Full Name: ring-box 1
• Alias Symbols: BA554C12.1;E3 ubiquitin-protein ligase RBX1;E3 ubiquitin-protein transferase RBX1;Protein ZYP;regulator of cullins 1;RING finger protein 75;ring-box 1, E3 ubiquitin protein ligase;RING-box protein 1;RNF75;ROC1;ZYP protein.
• NCBI Gene Id: 9978
• Protein Name: E3 ubiquitin-protein ligase RBX1
• Description of Target: E3 ubiquitin ligase component of multiple cullin-RING-based E3 ubiquitin-protein ligase (CRLs) complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins, including proteins involved in cell cycle progression, signal transduction, transcription and transcription-coupled nucleotide excision repair. CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (PubMed:27565346). The functional specificity of the E3 ubiquitin-protein ligase complexes depends on the variable substrate recognition components. As a component of the CSA complex promotes the ubiquitination of ERCC6 resulting in proteasomal degradation. Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme, like CDC34, to the complex and brings it into close proximity to the substrate. Probably also stimulates CDC34 autoubiquitination. May be required for histone H3 and histone H4 ubiquitination in response to ultraviolet and for subsequent DNA repair. Promotes the neddylation of CUL1, CUL2, CUL4 and CUL4 via its interaction with UBE2M. Involved in the ubiquitination of KEAP1, ENC1 and KLHL41. In concert with ATF2 and CUL3, promotes degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM.
• Uniprot ID: P62877
• Protein Accession #: NP_001158196.1
• Nucleotide Accession #: NM_001164724.1
• Protein Size (# AA): Recombinant
• Molecular Weight: 14 kDa