RBX1 Antibody - middle region : HRP (AVARP03042_T100-HRP)

Catalog No: AVARP03042_T100-HRP

Size:100ul

Product Info

• Publications:

  Maerki, S. et al. The Cul3-KLHL21 E3 ubiquitin ligase targets aurora B to midzone microtubules in anaphase and is required for cytokinesis. J. Cell Biol. 187, 791-800 (2009). WB, Bovine, Dog, Guinea pig, Human, Mouse, Rat, Zebrafish 19995937

  More...

• Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Zebrafish
• Product Format: Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: HRP: Horseradish Peroxidase
• Application: WB
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RBX1
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100%
• Peptide Sequence: Synthetic peptide located within the following region: NQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH
• Concentration: 0.5 mg/ml
• Blocking Peptide: For anti-RBX1 (AVARP03042_T100-HRP) antibody is Catalog # AAP30461 (Previous Catalog # AAPP01045)

Target Info

• Reference: Zheng, N., et al., (2002) Nature 416 (6882), 703-709.
• Gene Symbol: RBX1
• Gene Full Name: Ring-box 1, E3 ubiquitin protein ligase
• Alias Symbols: ROC1, RNF75, BA554C12.1
• NCBI Gene Id: 9978
• Description of Target: ROC1 encodes an evolutionarily conserved protein that interacts with cullins. The protein plays a unique role in the ubiquitination reaction by heterodimerizing with cullin-1 to catalyze ubiquitin polymerization. It also may be involved in the regulation of protein turn-over.
• Uniprot ID: P62877
• Protein Accession #: NP_055063
• Nucleotide Accession #: NM_014248
• Protein Size (# AA): 108
• Molecular Weight: 12kDa
• Protein Interactions: CDT1; CDC34; KEAP1; UBE2L6; UBE2M; CUL1; CUL3; CUL4B; UBE2D3; UBE2D2; UBE2D1; DAB2IP; UBC; TP53; PRAME; DCUN1D1; CUL2; DCUN1D3; MAP4K1; VHL; NEDD4; MTSS1; VRK2; HIST1H4A; GFPT1; CTNNB1; CCNE1; DCUN1D5; FBXL5; RHOBTB3; SAMHD1; UBC12; FBXL15; UBE2R2; SERTAD