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Catalog No: OPPA01659 (Formerly GWB-BDD311)
Size:2UG
Price: $75.00
SKU
OPPA01659
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RBP4 Protein (OPPA01659)

Datasheets/ManualsPrintable datasheet for OPPA01659
Product Info
Predicted Species ReactivityHuman
Product FormatRBP-4 His tag was filtered (0.4 um) and lyophilized from 0.5 mg/ml in PBS buffer, pH 7.5. Physical appearance: White lyophilized (freeze-dried) powder.
HostE. Coli
Additional InformationSolubility: It is recommended to reconstitute the lyophilized Retinol binding protein-4 in sterile deionized water to a working volume of 0.5mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile
::Biological Activity: Determined by its ability to bind all trans retinol acids. The binding of RBP4 to all trans retinol acids results in quenching of Trp fluroscence. 0.5M retinol acids/per M RBP4.
::Product Introduction: Retinol binding protein 4(RBP4) belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. This protein was found to be expressed and secreted by adipose tissue, and was strongly associated with insulin resistance. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli.RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the rbp-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.
Product Description: RBP-4 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 204 amino acids and having a molecular mass of 23 kDa.RBP4 is fused to N-terminus His tag which containes additional 21 amino acids. RBP4 sequence is identical to UniProtKB/Swiss-Prot entry Q5VY30 amino acids 16 - 199.The Retinol Binding Protein-4 is purified by proprietary chromatographic techniques.
Reconstitution and StorageLyophilized Retinol binding protein-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Retinol binding protein-4 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
PurityGreater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Peptide SequenceMSWWHHHHHH NWNIPTTQDT TERDCRVSSF RVKENFDKAR FSGTWYAMAK KDPEGLFLQD NIVAEFSVDE TGQMSATAKG RVRLLNNWDV CADMVGTFTD TEDPAKFKMK YWGVASFLQK GNDDHWIVDT DYDTYAVQYS CRLLNLDGTC ADSYSFVFSR DPNGLPPEAQ KIVRQRQEEL CLARQYRLIV HNGYCDGRSERNLL.
Gene SymbolRBP4 His
Alias SymbolsRetinol Binding Protein 4 plasma, RBP-4, RBP4, Plasma retinol-binding protein, PRBP, RBP, RBP4 Human, His, Retinol Binding Protein-4 Human Recombinant, His tag
Protein NamePlasma retinol-binding protein(1-182) Ensembl ENSP00000360524
Description of TargetRecombinant Human Retinol Binding Protein-4 His Tag
Uniprot IDQ5VY30
Protein Size (# AA)Recombinant
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