Catalog No: OPPA01659 (Formerly GWB-BDD311)
Size:2UG
Price: $75.00
SKU
OPPA01659
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/Manuals | Printable datasheet for OPPA01659 |
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Predicted Species Reactivity | Human |
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Product Format | RBP-4 His tag was filtered (0.4 um) and lyophilized from 0.5 mg/ml in PBS buffer, pH 7.5. Physical appearance: White lyophilized (freeze-dried) powder. |
Host | E. Coli |
Additional Information | Solubility: It is recommended to reconstitute the lyophilized Retinol binding protein-4 in sterile deionized water to a working volume of 0.5mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile |
:: | Biological Activity: Determined by its ability to bind all trans retinol acids. The binding of RBP4 to all trans retinol acids results in quenching of Trp fluroscence. 0.5M retinol acids/per M RBP4. |
:: | Product Introduction: Retinol binding protein 4(RBP4) belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. This protein was found to be expressed and secreted by adipose tissue, and was strongly associated with insulin resistance. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli.RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the rbp-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. Product Description: RBP-4 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 204 amino acids and having a molecular mass of 23 kDa.RBP4 is fused to N-terminus His tag which containes additional 21 amino acids. RBP4 sequence is identical to UniProtKB/Swiss-Prot entry Q5VY30 amino acids 16 - 199.The Retinol Binding Protein-4 is purified by proprietary chromatographic techniques. |
Reconstitution and Storage | Lyophilized Retinol binding protein-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Retinol binding protein-4 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles. |
Purity | Greater than 95.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Peptide Sequence | MSWWHHHHHH NWNIPTTQDT TERDCRVSSF RVKENFDKAR FSGTWYAMAK KDPEGLFLQD NIVAEFSVDE TGQMSATAKG RVRLLNNWDV CADMVGTFTD TEDPAKFKMK YWGVASFLQK GNDDHWIVDT DYDTYAVQYS CRLLNLDGTC ADSYSFVFSR DPNGLPPEAQ KIVRQRQEEL CLARQYRLIV HNGYCDGRSERNLL. |
Gene Symbol | RBP4 His |
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Alias Symbols | Retinol Binding Protein 4 plasma, RBP-4, RBP4, Plasma retinol-binding protein, PRBP, RBP, RBP4 Human, His, Retinol Binding Protein-4 Human Recombinant, His tag |
Protein Name | Plasma retinol-binding protein(1-182) Ensembl ENSP00000360524 |
Description of Target | Recombinant Human Retinol Binding Protein-4 His Tag |
Uniprot ID | Q5VY30 |
Protein Size (# AA) | Recombinant |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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