RBP4 ELISA Kit (Bovine) (OKEH07504)

Catalog No: OKEH07504

Size:96T

Bovine RBP4 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Bos taurus, Bovine
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology RBP4 ELISA Kit (Bovine) (OKEH07504) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RBP4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RBP4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RBP4 captured in well.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=8.4% (n = 20)
  Mean Inter-assay CV%: <=11.0% (n = 20)
• ELISA Kit Component:
  • RBP4 Microplate: 96 Wells (12 x 8 Well strips)
  • RBP4 Lyophilized Standard: 2
  • 100X Biotinylated RBP4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.
  Subcellular Location: Secreted
  Unigene: Bt.5318
  SMR: P18902
  String: 9913.ENSBTAP00000000566
  Pfam: PF00061
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.48ng/mL
• Specificity: Natural and recombinant Bovine Retinol-binding protein 4
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RBP4
• Alias Symbols: Plasma retinol-binding protein.
• Protein Name: P18902
• Description of Target: Retinol-binding protein 4
• Uniprot ID: https://www.uniprot.org/uniprot/P18902