RBP3 ELISA Kit (Human) (OKDD00505)

Catalog No: OKDD00505 (Formerly GWB-KBBFP9)

Size:96 Wells

Human RBP3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology RBP3 ELISA Kit (Human) (OKDD00505) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for RBP3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RBP3 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample RBP3 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RBP3 were tested 20 times on one plate, respectively.

  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RBP3 were tested on 3 different plates, 8 replicates in each plate.

  Intra-Assay: CV<10%

  Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-RBP3 Microplate: 96 Wells (12 x 8 Well Strips)
  • RBP3 Lyophlized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X RBP3 Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 10X Sample Diluent: 1 x 30 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates
• Sensitivity: < 0.051 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of RBP3. No significant cross-reactivity or interference between RBP3 and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RBP3
• Gene Full Name: retinol binding protein 3
• Alias Symbols: D10S64;D10S65;D10S66;interphotoreceptor retinoid-binding protein;interstitial retinol binding protein 3;Interstitial retinol-binding protein;IRBP;RBPI;retinol binding protein 3, interstitial;retinol-binding protein 3;RP66.
• NCBI Gene Id: 5949
• Protein Name: Retinol-binding protein 3
• Description of Target: IRBP shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina.
• Uniprot ID: P10745
• Protein Accession #: NP_002891.1
• Nucleotide Accession #: NM_002900.2
• Protein Size (# AA): 1247
• Molecular Weight: 135 kDa