RBP1 Chemi-Luminescent ELISA Kit (Human) (OKCD03901)

Catalog No: OKCD03901

Size:96WELLS

Human RBP1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-103%	78-94%	82-105%	95-104%
  EDTA Plasma (n=5)	88-95%	94-105%	97-104%	79-93%
  Heparin Plasma (n=5)	80-99%	97-105%	91-101%	84-99%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Renin (REN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Renin (REN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Renin (REN) level in the sample or standard.
• ELISA Kit Range: 5.49-4,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	91-99	95
  EDTA Plasma (n=5)	88-104	97
  Heparin Plasma (n=5)	79-96	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Renin (REN) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Renin (REN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • RBP1 Microplate: 96 Wells (12 x 8 Well strips)
  • RBP1 Lyophilized Standard: 2
  • 100X RBP1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.00549 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: REN
• Gene Full Name: renin
• Alias Symbols: ADTKD4;angiotensin-forming enzyme;angiotensinogenase;HNFJ2;renin;renin precursor, renal;RTD.
• NCBI Gene Id: 5972
• Protein Name: Renin
• Description of Target: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
• Uniprot ID: P00797
• Protein Accession #: NP_036865
• Nucleotide Accession #: NM_012733