RBP1 Chemi-Luminescent ELISA Kit (Human) (OKCD03900)

Catalog No: OKCD03900

Size:96WELLS

Human RBP1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	97-104%	92-101%	89-97%	78-104%
  EDTA Plasma (n=5)	96-103%	78-101%	84-94%	93-101%
  Heparin Plasma (n=5)	78-98%	78-90%	97-104%	79-104%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Retinol Binding Protein 1, Cellular (RBP1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Retinol Binding Protein 1, Cellular (RBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Retinol Binding Protein 1, Cellular (RBP1) level in the sample or standard.
• ELISA Kit Range: 0.27-200ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	98-105	101
  EDTA Plasma (n=5)	94-101	98
  Heparin Plasma (n=5)	93-102	98

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinol Binding Protein 1, Cellular (RBP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinol Binding Protein 1, Cellular (RBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • RBP1 Microplate: 96 Wells (12 x 8 Well strips)
  • RBP1 Lyophilized Standard: 2
  • 100X RBP1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 0.27 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RBP1
• Gene Full Name: retinol binding protein 1
• Alias Symbols: cellular retinol binding protein 1;Cellular retinol-binding protein;cellular retinol-binding protein I;CRABP-I;CRBP;CRBP1;CRBPI;RBPC;retinol-binding protein 1;retinol-binding protein 1, cellular.
• NCBI Gene Id: 5947
• Protein Name: Retinol-binding protein 1
• Description of Target: Cytoplasmic retinol-binding protein (PubMed:22665496, PubMed:26900151, PubMed:28057518). Accepts retinol from the transport protein STRA6, and thereby contributes to retinol uptake, storage and retinoid homeostasis (PubMed:15632377, PubMed:22665496).
• Uniprot ID: P09455
• Protein Accession #: NP_001124464
• Nucleotide Accession #: NM_001130992