RBM20 Antibody (OAEB01058)

Catalog No: OAEB01058 (Formerly GWB-EV1025)

Size:100UG

OAEB01058 (1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, P-IF
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: RBM20
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human, Mouse, Rat, Cow
• Peptide Sequence: C-QLDKAELDERP
• Concentration: 0.5 mg/ml
• Epitope: Internal region
• Application Info: P-ELISA: Antibody detection limit dilution 1:8000
  WB: Approx 150 kDa band observed in Mouse Heart lysates (calculated MW of 130 kDa according to Mouse NP_001164318.1). Recommended concentration: 1-3 ug/ml. Primary incubation 1 hour at room temperature. An additional band of 25 kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal.
  IF: Expression of the protein seen in the Nucleus and Golgi apparatus of U2OS cells. Recommended concentration: 10 ug/ml.
• Reference: Li D, Morales A, Gonzalez-Quintana J, Norton N, Siegfried JD, Hofmeyer M; Hershberger RE. Identification of novel mutations in RBM20 in patients with ; dilated cardiomyopathy.; Clin Transl Sci. 2010 Jun;3(3):90-7.; PMID: 20590677

Target Info

• Gene Symbol: Rbm20|RBM20
• Gene Full Name: RNA binding motif protein 20
• Alias Symbols: 1110018J23Rik;2010003H22Rik;AI646761;probable RNA-binding protein 20;RNA-binding protein 20;truncated RNA binding motif protein 20.
• NCBI Gene Id: 282996|309544|73713
• Protein Name: RNA-binding protein 20
• Uniprot ID: Q5T481
• Protein Accession #: NP_001127835.1
• Molecular Weight: 134