- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
| Datasheets/Manuals | Printable datasheet for anti-RB1 (OASG06294) antibody |
|---|
| Predicted Species Reactivity | Human, Mouse, Rat |
|---|---|
| Product Format | Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Application | WB, IHC, IF |
| Reconstitution and Storage | Store at -20C. Avoid repeated freeze/thaw cycles. |
| Immunogen | Synthesized peptide derived from human Rb around the non-phosphorylation site of S811. Location: 740-820aa. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration | 1 mg/ml |
| Specificity | Rb Polyclonal Antibody detects endogenous levels of Rb protein. |
| Application Info | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; |
| Gene Symbol | RB1 |
|---|---|
| Gene Full Name | retinoblastoma 1 |
| Alias Symbols | RB, pRb, OSRC, pp110, p105-Rb, PPP1R130, p110-RB1 |
| NCBI Gene Id | 5925; 19645; 24708 |
| Description of Target | The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. |
| Uniprot ID | P06400, P13405, P33568 |
| Molecular Weight | 89 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
-
What is the species homology for "RB1 Antibody (OASG06294)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat".
-
How long will it take to receive "RB1 Antibody (OASG06294)"?
This item is available "Domestic: within 2-3 weeks delivery | International: 2-3 weeks".
-
What buffer format is "RB1 Antibody (OASG06294)" provided in?
This item is provided in "Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "RB1 Antibody (OASG06294)"?
This target may also be called "RB, pRb, OSRC, pp110, p105-Rb, PPP1R130, p110-RB1" in publications.
-
What is the shipping cost for "RB1 Antibody (OASG06294)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
-
What is the guarantee for "RB1 Antibody (OASG06294)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
-
Can I get bulk pricing for "RB1 Antibody (OASG06294)"?
You can get bulk pricing for this item by going here.
-
What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "89 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "RB1 Antibody (OASG06294)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
-
What are positive controls for "RB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What are negative controls for "RB1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
-
What other proteins interact with "RB1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
-
What biological processes are associated with "RB1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
-
What cellular components are associated with "RB1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
-
What protein functions are associated with "RB1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
