Catalog No: OAAF01078 (Formerly GWB-ASC477)
Size:100 ug
Price: $344.00
SKU
OAAF01078
Availability: Domestic: within 1-2 week delivery | International: 1-2 weeks
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Datasheets/ManualsPrintable datasheet for RB1 Antibody (OAAF01078)
Product Info
Predicted Species ReactivityHuman|Mouse|Rat
Product FormatLiquid
ClonalityPolyclonal
HostRabbit
ApplicationEnzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin
Reconstitution and Storage-20°C
ImmunogenThe antiserum was produced against synthesized peptide derived from human Retinoblastoma.
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
SpecificityRetinoblastoma antibody detects endogenous levels of total Retinoblastoma protein.
FormulationRabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
DilutionThe optimal working dilution should be determined by the end user.
Application InfoIHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:1000
ReferenceIshizaka Y., Oncogene 4:789-794(1989).
Angrist M., Hum. Mol. Genet. 4:821-830(1995).
Sjoeblom T., Science 314:268-274(2006)
Gene SymbolRB1
Gene Full NameRB transcriptional corepressor 1
Alias Symbolsexon 17 tumor GOS561 substitution mutation causes premature stop;GOS563 exon 17 substitution mutation causes premature stop;OSRC;p105-Rb;p110-RB1;pp110;PPP1R130;pRb;prepro-retinoblastoma-associated protein;protein phosphatase 1, regulatory subunit 130;RB;retinoblastoma 1;retinoblastoma suspectibility protein;retinoblastoma-associated protein.
NCBI Gene Id5925
Protein NameRetinoblastoma-associated protein
Description of TargetKey regulator of entry into cell division that acts as a tumor suppressor. Promotes G0-G1 transition when phosphorylated by CDK3/cyclin-C. Acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Uniprot IDP06400
Protein Accession #NP_000312.2
Molecular Weight106 kDa
  1. What is the species homology for "RB1 Antibody (OAAF01078)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".

  2. How long will it take to receive "RB1 Antibody (OAAF01078)"?

    This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".

  3. What buffer format is "RB1 Antibody (OAAF01078)" provided in?

    This item is provided in "Liquid".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "RB1 Antibody (OAAF01078)"?

    This target may also be called "exon 17 tumor GOS561 substitution mutation causes premature stop;GOS563 exon 17 substitution mutation causes premature stop;OSRC;p105-Rb;p110-RB1;pp110;PPP1R130;pRb;prepro-retinoblastoma-associated protein;protein phosphatase 1, regulatory subunit 130;RB;retinoblastoma 1;retinoblastoma suspectibility protein;retinoblastoma-associated protein." in publications.

  5. What is the shipping cost for "RB1 Antibody (OAAF01078)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "RB1 Antibody (OAAF01078)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "RB1 Antibody (OAAF01078)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "106 kDa".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "RB1 Antibody (OAAF01078)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "RB1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "RB1"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "RB1"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "RB1"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "RB1"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "RB1"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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