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Rat TNF ALPHA Protein (OPSA11151)

20 ug
$459.00
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Gene Symbol:
Tnf
Alias Symbols:
Tnfa, RATTNF, MGC124630, TNF-alpha, Tnf
NCBI Gene Id:
24835
Swissprot Id:
P16599
Protein Accession #:
NP_036807.1
Protein Name:
Tumor necrosis factor
Description of Target:
RECOMBINANT RAT TNF ALPHA
Protein Size (# AA):
235
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Tnf.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Tnf.
Product Format:
Purified recombinant protein - lyophilised
Predicted Homology Based on Immunogen Sequence:
Rat
Predicted Species Reactivity:
Rat
Purity:
>98% by SDS PAGE and HPLC
Specificity:
Recombinant rat TNF alpha was tested in a cytotoxicity assay on murine L929 cells - this batch demonstrated an ED50 of < 0.05ng/ml.
Additional Information:
Activity: >2 x 107 units/mg
Approx Protein Conc: 0.5 mg/ml
Buffer solution: Phosphate buffered saline
:::
Endotoxin Level: < 0.1ng/ug
:::
Label Protein Molar Ratio: Recombinant rat TNF alpha purified from E.coli.
Preservative: None present
Reconstitution and Storage:
Reconstitution: Reconstitute with 40ul distilled water.
Storage: Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Target Reference:
1. Rayner, S. et al. (2000) Local bioactive tumour necrosis factor (TNF) in corneal allotransplantation. . Clin. Exp. Immunol 122: 109 - 116.
2. Lee, P. et al. (2000) Role of alveolar macrophages in innate immunity in neonates: evidence for selective lipopolysaccharide binding protein production by rat neonatal alveolar macrophages. Am. J. Respir. Cell. Mol. Biol. 23: 652 - 661.
Datasheets/Manuals:
Printable datasheet for OPSA11151

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