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Rat IgG2c Antibody : Cy5 (OASB00789)

100 ug
$410.00
In Stock
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Tested Species Reactivity:
Rat
Clonality:
Monoclonal
Clone:
2C8F1
Isotype:
IgG2a
Host:
Mouse
Conjugation:
CY5: Cyanine 5
Application:
FC, FLISA, IHC-F
Reconstitution and Storage:
Store at 2-8C
Official Gene Full Name:
Ig gamma-2C chain C region
Swissprot Id:
P20762
Immunogen:
Rat IgG2c hybridoma
Concentration:
0.1 mg/mL
Datasheets/Manuals:
Printable datasheet for OASB00789
Specificity:
IgG2c
Characterization:
To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested as a second step reagent byflow cytometry and/or ELISA to conform with characteristics of a standard reference reagent. Representativedata are included in this product insert.
Warning:
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eyeor face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact aphysician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds inrunning water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Dilution:
Flow Cytometry:
ELISA:
Fluorescein conjugate
CyTM 5 conjugate
Biotin conjugate
R-phycoerythrin conjugate
Horseradish Peroxidase conjugate
Alkaline Phosphatase conjugate
Biotin conjugate
<=1.0 ug/106 cells
<=0.1 ug/106 cells
<=1.0 ug/106 cells
<=0.1 ug/106 cells
1:1,000-1:4,000
1:1,000-1:4,000
1:5,000-1:10,000
Application Info:
Identification and enumeration of IgG2c+ cells by immunofluorescence microscopy, Second step reagent for rat IgG2c monoclonal antibodies, ELISA
Other Applications Data:
Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specificneeds.
Other Applications Image 1 Data:
Amount Used: 0.3 Ug/106 cells BALB/c splenocytes were incubated with rat IgG2a isotype, rat anti-mouse CD22 (2D6, rat IgG1), rat anti-mouse CD8 (53-6.7, rat IgG2a), rat anti-mouse CD45(LCA) (I3/2.3, rat IgG2b), rat anti-mouse CD24 (30-F1, rat IgG
Storage:
- The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg and the Cyanine 5 (CYTM5) is supplied as 0.1mg in 1.0 mL ofPBS/NaN3. Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mM MgCl2/50%Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Storeat 2- 8 C. Do not freeze!
- Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored asdirected.

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