Catalog No: OOSA10367 (Formerly GWB-5E3DCF)
Size:100T
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Rat IgG2a NEGATIVE CONTROL: RPE Negative/Isotype Control (OOSA10367)
Datasheets/Manuals | Printable datasheet for Rat IgG2a NEGATIVE CONTROL: RPE Negative/Isotype Control (OOSA10367) |
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Predicted Species Reactivity | Negative Control |
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Product Format | Lyophilized. Phosphate buffered saline |
Isotype | IgG2a |
Conjugation | R-PE |
Application | Flow cytometry |
Additional Information | Fusion Partner: Spleen cells from immunised DA rats were fused with cells of the rat Y3/Ag1.2.3 myeloma cell line. Immunogen: Human lymphocytes. |
Reconstitution and Storage | 2°C to 8°C |
Specificity | Rat IgG2a Negative Control antibody is suitable for the assessment of the level of non-specific binding of rat IgG2a monoclonal antibodies to mouse cells. Test results indicate Rat IgG2a Negative Control antibody is also suitable for use as a negative control with dog cells. This antibody recognizes a human cell surface marker, and therefore is not suitable as a negative control in human cells or cell lines. |
Application Info | Flow Cytometry Use 10ul of the suggested working dilution to label 106 cells in 100ul. |
Immunogen | Human lymphocytes. |
Purification | Protein G Purified |
Description of Target | RAT IgG2a NEGATIVE CONTROL:RPE |
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- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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