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Datasheets/Manuals | Printable datasheet for Mouse IL-2 Antibody : PE (OASB01243) |
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Tested Species Reactivity | Mouse |
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Predicted Species Reactivity | Mouse |
Clonality | Monoclonal |
Clone | JES6-5H4 |
Isotype | IgG2b |
Host | Rat |
Conjugation | R-PE |
Application | FC, MP |
Reconstitution and Storage | Store at 2-8C |
Immunogen | E. coli-expressed mouse IL-2 |
Concentration | 0.1 mg/mL |
Specificity | IL-2 |
Characterization | To ensure acceptable performance, each batch of product is tested in a sandwich ELISA and/or FlowCytometry to conform to characteristics of a standard reference reagent. |
Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact withskin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, washwith copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azideyields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running waterbefore discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
Dilution | Flow Cytometry: ELISA: Purified (UNLB) antibody FITC conjugate PE Conjugate BIOT conjugate <= 1 ug/106 cells 10 uL/106 cells 10 uL/106 cells 1:5,000-1:20,000 |
Application Info | ELISA: Purified JES6-5H4 monoclonal antibody may be used as capture antibody for quantifying mouse IL-2 protein levels. It should be paired with biotinylated JES6-1A12 (OASB01238) as detection antibody. Biotinylated JES6-5H4 is useful as a detection antibody and should be paired with purified JES6-1A12 antibody (OASB01237) as capture antibody. Flow Cytometry: FITC and R-PE conjugated JES6-5H4 antibodies are useful for intracellular staining in cells producing IL-2. Aviva intracellular staining buffers are recommended for this application. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. |
Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at2- 8 C - The R- phycoerythrin (R- PE) is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent Store at 2- 8 C. Do not freeze! - Reagents are stable for the period shown on the label if stored as directed. |
Reference | 1. Abrams, J.S., M.- G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti- cytokine monoclonalantibody development: Immunoassay of IL- 10 and IL- 5 in clinical samples. Immunol. Rev. 127:5- 24 2. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells inmurine peripheral blood and sera. J. Immunol. Meth. 166:201- 214 3. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP- labeled anti- cytokine antibodies. In CurrentProtocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit6.20. |
Gene Symbol | IL2 |
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Gene Full Name | Interleukin-2 |
Alias Symbols | IL, Il-2 |
NCBI Gene Id | 16183 |
Protein Name | interleukin-2 |
Uniprot ID | P04351 |
Protein Accession # | NP_032392.1 |
Nucleotide Accession # | NM_008366.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "Mouse IL-2 Antibody : PE (OASB01243)"?
The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "Mouse".
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How long will it take to receive "Mouse IL-2 Antibody : PE (OASB01243)"?
This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".
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What buffer format is "Mouse IL-2 Antibody : PE (OASB01243)" provided in?
This item is provided in "".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "Mouse IL-2 Antibody : PE (OASB01243)"?
This target may also be called "IL, Il-2" in publications.
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What is the shipping cost for "Mouse IL-2 Antibody : PE (OASB01243)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "Mouse IL-2 Antibody : PE (OASB01243)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "Mouse IL-2 Antibody : PE (OASB01243)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "Mouse IL-2 Antibody : PE (OASB01243)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "IL2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "IL2"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "IL2"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "IL2"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "IL2"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "IL2"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.