Size:100 ug
$275.00
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SKU
OASB01243
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Mouse IL-2 Antibody : PE (OASB01243)

Catalog#: OASB01243
Domestic: within 1 week delivery | International: 1 week
More Information
Tested Species Reactivity Mouse
Clonality Monoclonal
Clone JES6-5H4
Isotype IgG2b
Host Rat
Conjugation PE: Phycoerythrin
Application FC, MP
Reconstitution and Storage Store at 2-8C
Replacement Item This antibody may replace item sc-133118 from Santa Cruz Biotechnology.
Immunogen E. coli-expressed mouse IL-2
Concentration 0.1 mg/mL
Datasheets/Manuals Printable datasheet for anti-IL2 (OASB01243) antibody
Specificity IL-2
Characterization To ensure acceptable performance, each batch of product is tested in a sandwich ELISA and/or FlowCytometry to conform to characteristics of a standard reference reagent.
Warning Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact withskin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, washwith copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azideyields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running waterbefore discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Dilution Flow Cytometry:
ELISA:
Purified (UNLB) antibody
FITC conjugate
PE Conjugate
BIOT conjugate
<= 1 ug/106 cells
10 uL/106 cells
10 uL/106 cells
1:5,000-1:20,000
Application Info ELISA: Purified JES6-5H4 monoclonal antibody may be used as capture antibody for quantifying mouse IL-2 protein levels. It should be paired with biotinylated JES6-1A12 (OASB01238) as detection antibody. Biotinylated JES6-5H4 is useful as a detection antibody and should be paired with purified JES6-1A12 antibody (OASB01237) as capture antibody. Flow Cytometry: FITC and R-PE conjugated JES6-5H4 antibodies are useful for intracellular staining in cells producing IL-2. Aviva intracellular staining buffers are recommended for this application. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications.
Storage - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg labeled antibody in 1.0 mL of PBS/NaN3. Store at2- 8 C
- The R- phycoerythrin (R- PE) is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent
Store at 2- 8 C. Do not freeze!
- Reagents are stable for the period shown on the label if stored as directed.
Target Reference 1. Abrams, J.S., M.- G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Strategies of anti- cytokine monoclonalantibody development: Immunoassay of IL- 10 and IL- 5 in clinical samples. Immunol. Rev. 127:5- 24
2. Sander, B., I. Hoiden, U. Andersson, E. Moller, and J. Abrams. 1993. Similar frequencies and kinetics of cytokine producing cells inmurine peripheral blood and sera. J. Immunol. Meth. 166:201- 214
3. Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP- labeled anti- cytokine antibodies. In CurrentProtocols in Immunology (J. Coligamn, A. Kruisbeek, D. Margulies, E. Shevach, W. Strober, eds.). John Wiley and Sons, New York. Unit6.20.
Gene Symbol IL2
Official Gene Full Name Interleukin-2
Alias Symbols Il-2
NCBI Gene Id 16183
Protein Name interleukin-2
Swissprot Id P04351
Protein Accession # NP_032392.1
Nucleotide Accession # NM_008366.3
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express IL2
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express IL2
  1. What is the species homology for "Mouse IL-2 Antibody : PE (OASB01243)"?

    The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "".

  2. How long will it take to receive "Mouse IL-2 Antibody : PE (OASB01243)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "Mouse IL-2 Antibody : PE (OASB01243)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Mouse IL-2 Antibody : PE (OASB01243)"?

    This target may also be called "Il-2" in publications.

  5. What is the shipping cost for "Mouse IL-2 Antibody : PE (OASB01243)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Mouse IL-2 Antibody : PE (OASB01243)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Mouse IL-2 Antibody : PE (OASB01243)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Mouse IL-2 Antibody : PE (OASB01243)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "IL2"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "IL2"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "IL2"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "IL2"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "IL2"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "IL2"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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