Mouse CD54/ICAM-1 Antibody : APC (OASB00496)

Catalog No: OASB00496

Size:100UG

C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse CD54-APC.

C57BL/6 mouse splenocytes were stained with Rat Anti-Mouse CD54-APC and Rat Anti-Mouse NK1.1-FITC.

Product Info

• Tested Species Reactivity: Mouse
• Predicted Species Reactivity: Mouse
• Clonality: Monoclonal
• Clone: YN1/1.7.4
• Isotype: IgG2b
• Host: Rat
• Conjugation: APC
• Application: FC
• Additional Information: Description: CD54, a member of the immunoglobulin superfamily of cell surface receptors and also known as Intercellular Adhesion Molecule-1 (ICAM-1), is a type I transmembrane glycoprotein that mediates important cell/cell interactions. CD54 is expressed on endothelial cells, dendritic cells, keratinocytes, and lymphocytes. Its expression is upregulated by inflammatory mediators such as IFN-gamma, IL-1, TNF-alpha, lipopolysaccharide, and phorbol esters. Endothelial CD54 contributes to the extravasation of leukocytes from blood vessels, particularly in areas of inflammation. CD54 on antigen-presenting cells contributes to antigen-specific T-cell activation, presumably by enhancing interactions between T cell and antigen-presenting cells. These adhesion reactions are mediated via the binding of CD54 to its major ligands, LFA-1 and Mac-1.
• Reconstitution and Storage: Store at 2-8C
• Immunogen: Mouse NS-1 cells
• Concentration: 0.1 mg/mL
• Specificity: CD54
• Characterization: To insure lot- to- lot consistency, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent.
• Dilution: Flow Cytometry: Fluorescein conjugate
  Biotin conjugate
  PE and APC conjugates
  <=1 ug/10⁶ cells
  <=1 ug/10⁶ cells
  <= 0.2 ug/10⁶ cells
• Application Info: Flow cytometry 1,2, Immunohistochemistry (frozen sections) 4, Immunoprecipitation 1, In vitro blocking studies 1, Western blotting 3
• Other Applications Data: Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
• Storage: - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
  - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
  - The R- phycoerythrin (R- PE) and Allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
  - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C
  - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
• Reference: 1. Takei, F. 1985. J. Immunol. 134:1403
  2. Horley, K.J., C. Carpenito, B. Baker, F. Takei. 1989. EMBO. J. 8:2889
  3. Welder, Clayton A., D.H.S. Lee, F. Takei. 1993. J. Immunol. 150:2203
  4. Qin, Lan, et. al. 1996. J. Immunol. 157:5016.

Target Info

• Gene Symbol: ICAM1
• Gene Full Name: Intercellular adhesion molecule 1
• Alias Symbols: MAL, CD54, Icam, Ly-4, Ly-47, Icam-1, MALA-2
• NCBI Gene Id: 15894; 396750
• Protein Name: intercellular adhesion molecule 1
• Description of Target: This gene encodes an integral membrane protein that binds leukocyte adhesion protein LFA-1. It participates in the innate immune response.
• Uniprot ID: P13597, Q9MZU5
• Protein Accession #: NP_034623.1
• Nucleotide Accession #: NM_010493.3