Search Antibody, Protein, and ELISA Kit Solutions

Rat Anti-Mouse CD4/L3T4 Antibody : PE/CY5.5 (OASB00267)

100 ug
$320.00
In Stock
Request Bulk Order Quote

Gene Symbol:
CD4
NCBI Gene Id:
920
Official Gene Full Name:
T-cell surface glycoprotein CD4
Protein Name:
T-cell surface glycoprotein CD4
Swissprot Id:
P01730
Protein Accession #:
NP_000607.1
Nucleotide Accession #:
NM_000616.4
Replacement Item:
This antibody may replace item sc-1140 from Santa Cruz Biotechnology.
Host:
Rat
Clonality:
Monoclonal
Application:
FC
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CD4.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CD4.
Product Format:
R- phycoerythrin- Cyanine 5.5 (R- PE- CY5.5) Conjugate
Datasheets/Manuals:
Printable datasheet for anti-CD4 (OASB00267) antibody
Specificity:
CD4/L3T4a, Mr 52 kDa
Characterization:
To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of astandard reference reagent. Representative data are included in this package insert.
Warning:
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or faceprotection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physicianimmediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Dilution:
Flow Cytometry: Fluorescein and Biotin conjugates <= 1 ug/106 cells
R-phycoerythrin conjugate
Allophycocyanin conjugate
R-phycoerythrin conjugate-Texas Red®
Spectral Red conjugate
Cyanine 5 conjugate
R-phycoerythrin-Cyanine 5.5 conjugate
R-phycoerythrin-Cyanine 7 conjugate
APC-CY5.5 and APC-CY7 conjugates
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
Clone:
GK1.5
Isotype:
IgG2b
Application Info:
- Flow cytometry 1
- Immunohistochemistry (FS) 15
- Immunoprecipitation 1,2
- In vivo and in vitro depletion of CD4+ cells 6,7
- Fractionation of CD4+ cells14
Other Applications Data:
Since applications vary, you should determine the optimum working dilution forthe product that is appropriate for your specific need.
Storage:
- The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The allophycocyanin (APC) conjugate is supplied as as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- The Spectral Red (SPRD), R- phycoerythrin - Texas Red (R- PE- TXRD), R- phycoerythrin- Cyanine 7 (RPE- CY 7), R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5 Allophycocyanin- Cyanine 5.5 (APC- CY 5.5),and Allophycocyanin- Cyanine 7 (APC- CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C
- The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
Target Reference:
1. Dialynas, D. P., D. B. Wilde, P. Marrack, et al. 1983. Immunol. Rev. 74:29
2. Lakey, E. K., E. Margoliash, F. W. Fitch, and S. K. Pierce. 1986. J. Immunol. 136:3933
3. Frederickson, G.G., and R.S. Basch. 1989. J. Exp. Med. 1969:1473
4. Wineman, J.P., G.L. Gilmore, C. Gritzmacher, B.E. Torbett, and C.E. Muller- Sieburg. 1992. Blood 60:1717
5. Wu, L., R. Scollay, M. Egerton, M. Pearse, G.J. Spangrude, and K. Shortman. 1991. Nature 349:71
6. Hathcock, K.S. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1
7. Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1
8. Dialynas, D. P., Z. S. Quan, K. A. Wall, et al. 1983. J. Immunol. 131:2445
9. Swain, S. L., D. P. Dialynas, F. W. Fitch, and M. English. 1984. J. Immunol. 132:1118
10. Havran, W. L. and F. W. Fitch. 1988. J. Immunol. 141:1808
11. Bluestone, J.A., R.Q. Cron, T.A. Barrett, et al. 1991. Immunol. Rev. 120:5
12. Moldwin, R. L., G. J. Nau, W. L. Havran, D. W. Lancki, and F. W. Fitch. 1987. Ann. Inst. Pasteur Immunol. 138:131
13. Haque, S., K. Saizawa, J. Rojo, and C. A. Janeway, Jr. 1987. J. Immunol. 139:3207
14. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley and Sons, New York, p. 3.5.1
15. B. Zheng, B., S. Han, and G. Kelsoe. 1996. J. Exp. Med 184:1083- 1091.

Product Reviews

Tell us what you think about this item!

Write A Review
    Please, wait...