Size:100 ug
$335.00
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OASB00266
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Mouse CD4/L3T4 Antibody : CY5 (OASB00266)

Catalog#: OASB00266
Domestic: within 1 week delivery | International: 1 week
This product is Genway GWB-F8F27A
More Information
Tested Species Reactivity Mouse
Clonality Monoclonal
Clone GK1.5
Isotype IgG2b
Host Rat
Conjugation CY5: Cyanine 5
Application FC, IHC-F, ICC
Additional Information Description: CD4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested. CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors. The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells. In vivo and in vitro treatment with the GK1.5 monoclonal antibody effectively depletes CD4+ cells. GK1.5 also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.
Reconstitution and Storage Store at 2-8C
Replacement Item This antibody may replace item sc-1140 from Santa Cruz Biotechnology.
Immunogen Mouse CTL clone V4
Concentration 0.1 mg/mL
Datasheets/Manuals Printable datasheet for anti-CD4 (OASB00266) antibody
Specificity CD4
Characterization To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of astandard reference reagent. Representative data are included in this package insert.
Warning Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or faceprotection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physicianimmediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Dilution Flow Cytometry: Fluorescein and Biotin conjugates <= 1 ug/106 cells
R-phycoerythrin conjugate
Allophycocyanin conjugate
R-phycoerythrin conjugate-Texas Red®
Spectral Red conjugate
Cyanine 5 conjugate
R-phycoerythrin-Cyanine 5.5 conjugate
R-phycoerythrin-Cyanine 7 conjugate
APC-CY5.5 and APC-CY7 conjugates
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
<= 0.2 ug/106 cells
Application Info Flow cytometry 1, Immunohistochemistry (FS) 15, Immunoprecipitation 1,2, In vivo and in vitro depletion of CD4+ cells 6,7, Fractionation of CD4+ cells14
Other Applications Data Since applications vary, you should determine the optimum working dilution forthe product that is appropriate for your specific need.
Other Applications Image 1 Data Amount Used: 0.2 Ug/106 cells Cells from BALB/c mesenteric lymph nodes were double-stained with anti-mouse CD4-PE and anti-mouse CD8a-FITC , gated on small lymphocytes and analyzed by flow cytometry.
Storage - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The allophycocyanin (APC) conjugate is supplied as as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- The Spectral Red (SPRD), R- phycoerythrin - Texas Red (R- PE- TXRD), R- phycoerythrin- Cyanine 7 (RPE- CY 7), R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5 Allophycocyanin- Cyanine 5.5 (APC- CY 5.5),and Allophycocyanin- Cyanine 7 (APC- CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C
- The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
Target Reference 1. Dialynas, D. P., D. B. Wilde, P. Marrack, et al. 1983. Immunol. Rev. 74:29
2. Lakey, E. K., E. Margoliash, F. W. Fitch, and S. K. Pierce. 1986. J. Immunol. 136:3933
3. Frederickson, G.G., and R.S. Basch. 1989. J. Exp. Med. 1969:1473
4. Wineman, J.P., G.L. Gilmore, C. Gritzmacher, B.E. Torbett, and C.E. Muller- Sieburg. 1992. Blood 60:1717
5. Wu, L., R. Scollay, M. Egerton, M. Pearse, G.J. Spangrude, and K. Shortman. 1991. Nature 349:71
6. Hathcock, K.S. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1
7. Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1
8. Dialynas, D. P., Z. S. Quan, K. A. Wall, et al. 1983. J. Immunol. 131:2445
9. Swain, S. L., D. P. Dialynas, F. W. Fitch, and M. English. 1984. J. Immunol. 132:1118
10. Havran, W. L. and F. W. Fitch. 1988. J. Immunol. 141:1808
11. Bluestone, J.A., R.Q. Cron, T.A. Barrett, et al. 1991. Immunol. Rev. 120:5
12. Moldwin, R. L., G. J. Nau, W. L. Havran, D. W. Lancki, and F. W. Fitch. 1987. Ann. Inst. Pasteur Immunol. 138:131
13. Haque, S., K. Saizawa, J. Rojo, and C. A. Janeway, Jr. 1987. J. Immunol. 139:3207
14. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley and Sons, New York, p. 3.5.1
15. B. Zheng, B., S. Han, and G. Kelsoe. 1996. J. Exp. Med 184:1083- 1091.
Gene Symbol CD4
Official Gene Full Name T-cell surface glycoprotein CD4
Alias Symbols L3T4, Ly-4
NCBI Gene Id 12504
Protein Name T-cell surface glycoprotein CD4
Swissprot Id P06332
Protein Accession # NP_038516.1
Nucleotide Accession # NM_013488.2
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express CD4
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express CD4
  1. What is the species homology for "Mouse CD4/L3T4 Antibody : CY5 (OASB00266)"?

    The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "".

  2. How long will it take to receive "Mouse CD4/L3T4 Antibody : CY5 (OASB00266)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "Mouse CD4/L3T4 Antibody : CY5 (OASB00266)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Mouse CD4/L3T4 Antibody : CY5 (OASB00266)"?

    This target may also be called "L3T4, Ly-4" in publications.

  5. What is the shipping cost for "Mouse CD4/L3T4 Antibody : CY5 (OASB00266)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Mouse CD4/L3T4 Antibody : CY5 (OASB00266)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Mouse CD4/L3T4 Antibody : CY5 (OASB00266)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Mouse CD4/L3T4 Antibody : CY5 (OASB00266)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "CD4"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "CD4"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "CD4"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "CD4"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "CD4"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "CD4"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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