Size:100 ug
$370.00
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SKU
OASB00389
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Mouse CD25/IL-2Ra Antibody : APC (OASB00389)

Catalog#: OASB00389
Domestic: within 1 week delivery | International: 1 week
This product is Genway GWB-7D52E3
More Information
Tested Species Reactivity Mouse
Clonality Monoclonal
Clone 7D4
Isotype IgM
Host Rat
Conjugation APC: Allophycocyanin
Application FC
Additional Information Description: The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail. CD25 is expressed on activated T cells, B cells, NK cells, and monocytes of all mouse strains tested. It is transiently expressed at a low level during B cell development in bone marrow on the CD45R/B220low TdT- sIg- pre-B II and CD45R/B220low TdT- sIgM+sIgD- immature B cells, but not on the CD45R/B220low TdT+ sIg- pro-B/pre-B I stage nor on CD45R/B220high TdT- sIgM+sIgD+ mature B cells. CD25 is expressed at a higher level during very early T-cell development in fetal and adult thymus. The 7D4 monoclonal antibody reacts with an epitope between amino acids 125 and 212 that is distinct from that recognized by the monoclonal antibody 3C7 . While the monoclonal antibody 7D4 does not block the binding of IL-2 to CD25, when used in combination with the monoclonal antibody 3C7 it results in higher levels of inhibition of IL-2-driven proliferation than either of these two monoclonal antibodies alone.
Reconstitution and Storage Store at 2-8C
Replacement Item This antibody may replace item sc-13946 from Santa Cruz Biotechnology.
Immunogen IL-2 dependent BALB/c helper T cell clone HT2
Concentration 0.1 mg/mL
Datasheets/Manuals Printable datasheet for anti-IL2RA (OASB00389) antibody
Specificity CD25
Characterization To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conformto characteristics of a standard reference reagent. Representative data are included in this product insert.
Warning Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact withskin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, washwith copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azideyields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running waterbefore discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Dilution Flow Cytometry: Fluorescein conjugate <=1 ug/106 cells
Biotin conjugate <= 1 ug/106 cells
R-phycoerythrin conjugate <= 0.2 ug/106 cells
Allophycocyanin conjugate <= 0.2 ug/106 cells
Spectral Red conjugate <= 0.2 ug/106 cells
R-phycoerythrin-Cyanine 7 conjugate <= 0.2 ug/106 cells
Application Info Flow cytometry 1, 3, 4, 7, 8, Immunoprecipitation 1, 3
Other Applications Data Since applications vary, you should determine the optimum workingdilution for the product that is appropriate for your specific need.
Other Applications Image 1 Data Amount Used: 0.1 ug/106 cells Cells from BALB/c spleen were activated by PMA (10 ng/ml) and ionomycin (400 ng/ml) for 48 hours, then stained with anti-CD25-FITC and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA).
Storage - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 7 (R- PE- CY 7) conjugates are supplied as0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze!
- The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C
- Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected.
Target Reference 1. Malek, T. R., R. J. Robb, and E. M. Shevach. 1983. Proc. Natl. Acad. Sci. U. S. A. 80:5694
2. Leonard, W.J., et al. 1984. Nature 311:626
3. Ortega, G., R. J. Robb, E. M. Shevach, and T. R. Malek. 1984. J. Immunol. 133:1970
4. Malek, T. R., J. A. Schmidt, and E. M. Shevach. 1985. J. Immunol. 134:2405
5. Rolink, A., U. Grawunder, T.H. Winkler, H. Karasuyama, and F. Melchers. 1994. Int. Immunol. 6:1257
6. Chen, J., A. Ma, F. Young, and F. W. Alt. 1994. International. Immunology 6:1265
7. Habu, S., K. Okumura, T. Diamantstein, and E. M. Shevach. 1985. Eur. J. Immunol. 15:456
8. Moreau, J. L., M. Nabholz, T. Diamantstein, T. R. Malek, E. Shevach, and J. Theze. 1987. Eur. J. Immunol. 17:929
9. Lorenzo, F., C. Jaulin, N. Vita., P. Froussard, P. Ferrara, D. Jankovic , and J. Theze. J. Immunol. 147:2970.
Gene Symbol IL2RA
Official Gene Full Name Interleukin-2 receptor subunit alpha
Alias Symbols CD25, Il2r, Ly-43
NCBI Gene Id 16184
Protein Name interleukin-2 receptor subunit alpha
Swissprot Id P01590
Protein Accession # NP_032393.3
Nucleotide Accession # NM_008367.3
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express IL2RA
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express IL2RA
  1. What is the species homology for "Mouse CD25/IL-2Ra Antibody : APC (OASB00389)"?

    The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "".

  2. How long will it take to receive "Mouse CD25/IL-2Ra Antibody : APC (OASB00389)"?

    This item is available "Domestic: within 1 week delivery | International: 1 week".

  3. What buffer format is "Mouse CD25/IL-2Ra Antibody : APC (OASB00389)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Mouse CD25/IL-2Ra Antibody : APC (OASB00389)"?

    This target may also be called "CD25, Il2r, Ly-43" in publications.

  5. What is the shipping cost for "Mouse CD25/IL-2Ra Antibody : APC (OASB00389)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "Mouse CD25/IL-2Ra Antibody : APC (OASB00389)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Mouse CD25/IL-2Ra Antibody : APC (OASB00389)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Mouse CD25/IL-2Ra Antibody : APC (OASB00389)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data tab on the product page.

  10. What are positive controls for "IL2RA"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "IL2RA"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "IL2RA"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "IL2RA"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "IL2RA"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "IL2RA"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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