RASGRP2 Antibody - N-terminal region (OAAB08424)

Catalog No: OAAB08424

Size:400UL

RASGRP2 Antibody (N-term) (Cat. #OAAB08424) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RASGRP2 antibody detected the RASGRP2 protein (arrow).

RASGRP2 Antibody (N-term) (Cat. #OAAB08424)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RASGRP2 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of RASGRP2 Antibody (N-term)(Cat#OAAB08424) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: IF,IHC-P,WB
• Additional Information: Precautions: RASGRP2 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway
  Cellular Location: Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Cell junction, synapse, synaptosome (Potential). Cell projection, ruffle membrane; Peripheral membrane protein (Potential). Note=Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to th
  Tissue Location: Detected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This RASGRP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 7-34 amino acids from the N-terminal region of human RASGRP2.
• Predicted Homology Based on Immunogen Sequence: Cow; Human; Mouse; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  RASGRP2 is supported by BioGPS gene expression data to be expressed in K562

• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  IF: 1:10~50

Target Info

• Gene Symbol: RASGRP2
• Alias Symbols: calcium and DAG-regulated guanine nucleotide exchange factor I, calcium and diacylglycerol-regulated guanine nucleotide exchange factor I, calcium diacylglycerol guanine nucleotide exchange factor I, CalDAG-, CALDAG-GEFI, Caldagg, Caldaggef1, cdc25-like protein, CDC25L, F25B3.3 kinase like protein, F25B3.3 kinase-like protein, guanine exchange factor MCG7, RAP 1A protein-specific guanine nucleotide exchange factor 1, RAS guanyl nucleotide-releasing protein 2, RAS guanyl releasing protein 2 (calcium and DAG-regulated), RAS guanyl-releasing protein 2.
• NCBI Gene Id: 10235|19395|361714|512056
• Protein Name: RAS guanyl-releasing protein 2|RAS guanyl-releasing protein 2 isoform a [Homo sapiens]
• Description of Target: Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
• Uniprot ID: A6N9I4|P0C643|Q7LDG7|Q9QUG9
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_001092140.1,NP_001092141.1,NP_722541.1
• Molecular Weight: 69248