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| Datasheets/Manuals | Printable datasheet for RASGRF1 Antibody (Phospho-Ser916) (OAAF00135) |
|---|
| Predicted Species Reactivity | Mouse|Rat |
|---|---|
| Product Format | Liquid |
| Clonality | Polyclonal |
| Host | Rabbit |
| Application | Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot |
| Reconstitution and Storage | -20°C |
| Immunogen | The antiserum was produced against synthesized peptide derived from mouse Ras-GRF1 around the phosphorylation site of Ser916. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Specificity | Ras-GRF1 (Phospho-Ser916) antibody detects endogenous levels of Ras-GRF1 only when phosphorylated at serine 916. |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Dilution | The optimal working dilution should be determined by the end user. |
| Application Info | IHC: 1:50~1:100; ELISA: 1:20000 |
| Reference | Huibin Yang, J. Biol. Chem., Apr 2003; 278: 13278 - 13285. Huibin Yang, Mol. Biol. Cell, May 2006; 17: 2177 - 2189. Raymond R. Mattingly, J. Biol. Chem., Dec 1999; 274: 37379. Tie-Nian Zhu, J. Biol. Chem., May 2007; 282: 14816 - 14826. |
| Gene Symbol | Rasgrf1 |
|---|---|
| Gene Full Name | RAS protein-specific guanine nucleotide-releasing factor 1 |
| Alias Symbols | AI844718;CDC25;CDC25Mm;Gnrp;Grf;Grf1;Grfbe;Grfbeta;Guanine nucleotide-releasing protein;p190;P190-A;p190RhoGEF;Ras guanine release factor beta;Ras-GRF1;ras-specific guanine nucleotide-releasing factor 1;ras-specific nucleotide exchange factor CDC25. |
| NCBI Gene Id | 19417 |
| Protein Name | Ras-specific guanine nucleotide-releasing factor 1 |
| Description of Target | Promotes the exchange of Ras-bound GDP by GTP. |
| Uniprot ID | P27671 |
| Protein Accession # | NP_722522.1 |
| Molecular Weight | 145 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "RASGRF1 Antibody (Phospho-Ser916) (OAAF00135)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Mouse|Rat".
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How long will it take to receive "RASGRF1 Antibody (Phospho-Ser916) (OAAF00135)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "RASGRF1 Antibody (Phospho-Ser916) (OAAF00135)" provided in?
This item is provided in "Liquid".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "RASGRF1 Antibody (Phospho-Ser916) (OAAF00135)"?
This target may also be called "AI844718;CDC25;CDC25Mm;Gnrp;Grf;Grf1;Grfbe;Grfbeta;Guanine nucleotide-releasing protein;p190;P190-A;p190RhoGEF;Ras guanine release factor beta;Ras-GRF1;ras-specific guanine nucleotide-releasing factor 1;ras-specific nucleotide exchange factor CDC25." in publications.
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What is the shipping cost for "RASGRF1 Antibody (Phospho-Ser916) (OAAF00135)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "RASGRF1 Antibody (Phospho-Ser916) (OAAF00135)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "RASGRF1 Antibody (Phospho-Ser916) (OAAF00135)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "145 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "RASGRF1 Antibody (Phospho-Ser916) (OAAF00135)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "Rasgrf1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "Rasgrf1"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "Rasgrf1"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "Rasgrf1"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "Rasgrf1"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "Rasgrf1"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
