RARS Antibody - C-terminal region (OAAB07329)

Catalog No: OAAB07329

Size:400ul

RARS Antibody (C-term) (Cat. #OAAB07329) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the RARS antibody detected the RARS protein (arrow).

RARS Antibody (C-term) (Cat. #OAAB07329)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RARS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of RARS Antibody (C-term)(Cat#OAAB07329) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

RARS Antibody (C-term) (Cat. #OAAB07329) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, IF, FC
• Additional Information: Precautions: RARS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Forms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1
  Cellular Location: Cytoplasm.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This RARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 605-634 amino acids from the C-terminal region of human RARS.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  RARS is strongly supported by BioGPS gene expression data to be expressed in MCF7

• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  IF: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: RARS
• Alias Symbols: HLD9, RARS, ArgRS, DALRD1
• NCBI Gene Id: 5917
• Protein Name: Arginine--tRNA ligase, cytoplasmic
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: P54136
• Protein Accession #: NP_002878.2
• Molecular Weight: 75379 Da