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Catalog No: OKCD00112 (Formerly GWB-KBBVV4)
Size:96WELLS
Price: $650.00
SKU
OKCD00112
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Datasheets/ManualsPrintable datasheet for RARRES2 ELISA Kit (Rat) (OKCD00112)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)85-104%97-105%80-102%83-95%
EDTA plasma(n=5)88-95%81-98%88-101%87-95%
heparin plasma(n=5)83-103%93-101%96-104%85-98%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemerin (CHEM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemerin (CHEM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemerin (CHEM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chemerin (CHEM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range15.6-1,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)85-9289
EDTA plasma(n=5)91-9994
heparin plasma(n=5)86-10195
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemerin (CHEM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemerin (CHEM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Rarres2 Microplate96 Wells (12 x 8 Well Strips)
Rarres2 Lyophilized Standard2 x 1 ng
100X Biotinylated Rarres2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 6.9 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Chemerin (CHEM). No significant cross-reactivity or interference between Chemerin (CHEM) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolRarres2
Gene Full Nameretinoic acid receptor responder 2
Alias Symbolschemerin;retinoic acid receptor responder (tazarotene induced) 2;retinoic acid receptor responder protein 2.
NCBI Gene Id297073
Protein NameRetinoic acid receptor responder (Tazarotene induced) 2, isoform CRA_a
Description of TargetRetinoic acid receptor responder (Tazarotene induced) 2, isoform CRA_a
Uniprot IDQ5BK77
Protein Accession #NP_001013445
Nucleotide Accession #NM_001013427.1
Protein Size (# AA)163
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