RARB ELISA Kit (Human) (OKCD08572)

Catalog No: OKCD08572

Size:96WELLS

RARB ELISA Kit (Human) (OKCD08572) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoic Acid Receptor Beta (RARb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Retinoic Acid Receptor Beta (RARb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Retinoic Acid Receptor Beta (RARb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Retinoic Acid Receptor Beta (RARb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Retinoic Acid Receptor Beta (RARb) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Retinoic Acid Receptor Beta (RARb) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • RARB Microplate: 96 Wells (12 x 8 Well strips)
  • RARB Lyophilized Standard: 2
  • 100X RARB HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.054ng/mL

Target Info

• Gene Symbol: RARB
• Gene Full Name: retinoic acid receptor beta
• Alias Symbols: HAP;HBV-activated protein;hepatitis B virus activated protein;MCOPS12;NR1B2;nuclear receptor subfamily 1 group B member 2;RAR-beta;RARbeta1;RAR-epsilon;retinoic acid receptor beta;retinoic acid receptor, beta polypeptide;RRB2.
• NCBI Gene Id: 5915
• Protein Name: Retinoic acid receptor beta
• Description of Target: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
• Uniprot ID: P10826
• Protein Accession #: NP_000956.2
• Nucleotide Accession #: NM_000965.4