RARB ELISA Kit (Chicken) (OKEH07631)

Catalog No: OKEH07631

Size:96T

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology RARB ELISA Kit (Chicken) (OKEH07631) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RARB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RARB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RARB captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=2.6% (n = 20)
  Mean Inter-assay CV%: <=7.3% (n = 20)
• ELISA Kit Component:
  • RARB Microplate: 96 Wells (12 x 8 Well strips)
  • RARB Lyophilized Standard: 2
  • 100X Biotinylated RARB Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5 (By similarity). Required for limb and craniofacial development.
  Subcellular Location: Nucleus
  Unigene: Gga.2668
  SMR: P22448
  String: 9031.ENSGALP00000036953
  KEGG: gga:396266
  Pfam: PF00104
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Chicken Retinoic acid receptor beta
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RARB
• Gene Full Name: retinoic acid receptor beta
• Alias Symbols: nuclear receptor subfamily 1 group B member 2;RAR-beta;RARBETA;retinoic acid receptor beta;retinoic acid receptor beta 2;retinoic acid receptor beta4';retinoic acid receptor beta4M;retinoic acid receptor beta4M'.
• NCBI Gene Id: 396266
• Protein Name: P22448
• Description of Target: Retinoic acid receptor beta
• Uniprot ID: https://www.uniprot.org/uniprot/P22448
• Protein Accession #: NP_990657.1
• Nucleotide Accession #: NM_205326.1