Catalog No: OCOA00307
Size:20UG
Price: $179.00
SKU
OCOA00307
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RARA Protein Lysate (OCOA00307)

Datasheets/ManualsPrintable datasheet for RARA Protein Lysate (OCOA00307)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionRARA HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolRARA
Gene Full Nameretinoic acid receptor alpha
Alias SymbolsNR1B1;nuclear receptor subfamily 1 group B member 1;nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form;PML-DDX5-RARA fusion;PML-DDX5-RARA fusion protein;RAR;RAR-alpha;retinoic acid nuclear receptor alpha variant 1;retinoic acid nuclear receptor alpha variant 2;retinoic acid receptor alpha;retinoic acid receptor, alpha polypeptide.
NCBI Gene Id5914
Protein NameNM_000964, NP_000955, P10276
Description of TargetHomo sapiens retinoic acid receptor alpha (RARA), transcript variant 1, mRNA, Retinoic acid receptor alpha, retinoic acid receptor alpha isoform 1 [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P10276
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_000955
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_000964
Molecular Weight50.6 kDa
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