RARA Antibody - C-terminal region (OAAB10147)

Catalog No: OAAB10147

Size:400ul

RARA Antibody (C-term) (Cat. #OAAB10147) western blot analysis in HL-60 cell line and mouse testis tissue lysates (35ug/lane).This demonstrates the RARA antibody detected the RARA protein (arrow).

RARA Antibody (C-term) (Cat. #OAAB10147)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RARA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of RARA Antibody (C-term) (Cat#OAAB10147) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

RARA Antibody (C-term) (Cat. #OAAB10147) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IF, IHC-P, FC
• Additional Information: Cellular Location: Nucleus. Cytoplasm.
  Tissue Location: Expressed in monocytes.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This RARA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-349 amino acids from the C-terminal region of human RARA.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Varies by lot. See vial for concentration.
• Intended Use: For research use only.
• Application Info: IF: 1:10~50
  WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: RARA
• Alias Symbols: RAR, NR1B1
• NCBI Gene Id: 5914
• Protein Name: Retinoic acid receptor alpha
• Description of Target: This gene represents a nuclear retinoic acid receptor. The encoded protein, retinoic acid receptor alpha, regulates transcription in a ligand-dependent manner. This gene has been implicated in regulation of development, differentiation, apoptosis, granulopoeisis, and transcription of clock genes. Translocations between this locus and several other loci have been associated with acute promyelocytic leukemia. Alternatively spliced transcript variants have been found for this locus.
• Uniprot ID: P10276
• Protein Accession #: NP_000955.1
• Molecular Weight: 51 kDa