RARA Antibody - C-terminal region (OAAB10147)

Catalog No: OAAB10147

Size:400UL

RARA Antibody (C-term) (Cat. #OAAB10147) western blot analysis in HL-60 cell line and mouse testis tissue lysates (35ug/lane).This demonstrates the RARA antibody detected the RARA protein (arrow).

RARA Antibody (C-term) (Cat. #OAAB10147)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RARA Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of RARA Antibody (C-term) (Cat#OAAB10147) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

RARA Antibody (C-term) (Cat. #OAAB10147) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: Flow cytometry, Immunofluorescence, Immunohistochemistry-Paraffin, Western blot
• Additional Information: Cellular Location: Nucleus. Cytoplasm.
  Tissue Location: Expressed in monocytes.
• Reconstitution and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This RARA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-349 amino acids from the C-terminal region of human RARA.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Varies by lot. See vial for concentration.
• Intended Use: For research use only.
• Application Info: IF: 1:10~50
  WB: 1:1000
  IHC-P: 1:10~50
  FC: 1:10~50

Target Info

• Gene Symbol: RARA
• Gene Full Name: retinoic acid receptor alpha
• Alias Symbols: NR1B1;nuclear receptor subfamily 1 group B member 1;nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form;PML-DDX5-RARA fusion;PML-DDX5-RARA fusion protein;RAR;RAR-alpha;retinoic acid nuclear receptor alpha variant 1;retinoic acid nuclear receptor alpha variant 2;retinoic acid receptor alpha;retinoic acid receptor, alpha polypeptide.
• NCBI Gene Id: 5914
• Protein Name: NP_000955.1, NP_001019980.1, NP_001138773.1, NP_001138774.1, P10276
• Description of Target: Retinoic acid receptor alpha, retinoic acid receptor alpha isoform 1 [Homo sapiens], retinoic acid receptor alpha isoform 2 [Homo sapiens], retinoic acid receptor alpha isoform 4 [Homo sapiens]
• Uniprot ID: https://www.uniprot.org/uniprot/P10276
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_000955.1,NP_001019980.1,NP_001138773.1,NP_001138774.1
• Molecular Weight: 50771