RAPH1 ELISA Kit (Human) (OKEH08548)

Catalog No: OKEH08548

Size:96T

Human RAPH1 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology RAPH1 ELISA Kit (Human) (OKEH08548) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RAPH1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RAPH1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RAPH1 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 116% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.9% (n = 20)
  Mean Inter-assay CV%: <=6.6% (n = 20)
• ELISA Kit Component:
  • RAPH1 Microplate: 96 Wells (12 x 8 Well strips)
  • RAPH1 Lyophilized Standard: 2
  • 100X Biotinylated RAPH1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Mediator of localized membrane signals. Implicated in the regulation of lamellipodial dynamics. Negatively regulates cell adhesion.
  Subcellular Location: Cell membrane Peripheral membrane protein Cytoplasmic side Cell projection Lamellipodium Cell projection Filopodium Cytoplasm Cytoskeleton Recruited to the membrane, via the PH domain, by the phosphoinositide, PI(3,4)P2. Colocalizes with ENAH/VASP at the tips of lamellipodia and filopodia. Also colocalizes with the pathogens, Vaccinia and Enteropathogenic E.coli (EPEC) at the interface between the pathogen and their actin.
  Unigene: Hs.471162
  SMR: Q70E73
  String: 9606.ENSP00000316543
  KEGG: hsa:65059
  MIM: 609035
  Pfam: PF00169
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 38pg/mL
• Specificity: Natural and recombinant Human Ras-associated and pleckstrin homology domains-containing protein 1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RAPH1
• Gene Full Name: Ras association (RalGDS/AF-6) and pleckstrin homology domains 1
• Alias Symbols: ALS2CR18;ALS2CR9;amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 18;amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 9;Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 18 protein;Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 9 protein;lamellipodin;LPD;PREL2;PREL-2;proline-rich EVH1 ligand 2;Protein RMO1;RalGDS/AF-6;ras-associated and pleckstrin homology domains-containing protein 1;RMO1.
• NCBI Gene Id: 65059
• Protein Name: Q70E73
• Description of Target: Ras-associated and pleckstrin homology domains-containing protein 1
• Uniprot ID: https://www.uniprot.org/uniprot/Q70E73
• Protein Accession #: NP_976241.1
• Nucleotide Accession #: NM_203365.3