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Rantes ELISA Kit (Rat) (OKBB00245)

96 Tests
$575.00
In Stock
Application:
ELISA
Gene Symbol:
CCL5
Official Gene Full Name:
C-C motif chemokine ligand 5
NCBI Gene Id:
81780
Alias Symbols:
CCL5, RANTES, Chemokine CC Motif Ligand 5, SCYA5
Description of Target:
Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES.1 The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.2
Protein Name:
C-C motif chemokine 5
Swissprot Id:
P50231
Species Reactivity:
Rat
Sample Type:
cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Sensitivity:
< 1 pg/mL
Kit Range:
15.6 pg/mL-1,000 pg/mL
Kit Reproducibility:
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (pg/mL)135327536118293625
St. Dev.6.211729.489.6819.6343.75
%CV4.65.195.58.206.697
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology CCL5 ELISA Kit (Rat) (OKBB00245) is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody specific for CCL5 has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (E.coli, S24-S91) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CCL5 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Rat CCL5 captured in well.
Kit Component:
ComponentAmount
Lyophilized recombinant human MMP-7 standard10 ng/tube x 2
Anti-human MMP-7 Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti- human MMP-7 antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CCL5.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CCL5.
Predicted Homology Based on Immunogen Sequence:
No detectable cross-reactivity with any other cytokine.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Target Reference:
1. Liu, H.; Chao, D.; Nakayama, E. E.; Taguchi, H.; Goto, M.; Xin, X.; Takamatsu, J.; Saito, H.; Ishikawa, Y.; Akaza, T.;
Juji, T.; Takebe, Y.; and 9 others. Polymorphism in RANTES chemokine promoter affects HIV-1 disease progression.
Proc. Nat. Acad. Sci. 96: 4581-4585, 1999.
2. Schall, T. J.; Jongstra, J.; Dyer, B. J.; Jorgensen, J.; Clayberger, C.; Davis, M. M.; Krensky, A. M. A human T
cell-specific molecule is a member of a new gene family. J. Immun. 141: 1018-1025, 1988.
Specificity:
There is no detectable cross-reactivity with other relevant proteins.
Additional Information:
Range: 15.6pg/ml-1000pg/ml
:::
Principle: Aviva’s rat Rantes ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat Rantes specific-specific polyclonal antibodies were precoated onto 96-well plates. The rat specific detection monoclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Rantes amount of sample captured in plate.
:::
Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: Rantes (regulated on activation normal T cell expressed and secreted) is one of the natural ligands for the chemokine receptor CCR5 and potently suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Peripheral blood mononuclear cells or CD4(+) lymphocytes obtained from different individuals have wide variations in their ability to secrete RANTES.1 The Rantes gene product is predicted to be 10 kDa and, after cleavage of the signal peptide, approximately 8 kDa. Of the 68 residues, 4 are cysteines, and there are no sites for N-linked glycosylation. Rantes is expressed by cultured T cell lines that are Ag specific and growth factor dependent.2
Protocol:
Tips Information:

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