RAN ELISA Kit (Human) (OKEH08284)

Catalog No: OKEH08284

Size:96T

Human RAN ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology RAN ELISA Kit (Human) (OKEH08284) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RAN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RAN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RAN captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 94% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.6% (n = 20)
  Mean Inter-assay CV%: <=7.1% (n = 20)
• ELISA Kit Component:
  • RAN Microplate: 96 Wells (12 x 8 Well strips)
  • RAN Lyophilized Standard: 2
  • 100X Biotinylated RAN Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules (PubMed:18591255). Acts as a negative regulator of the kinase activity of VRK1 and VRK2 (PubMed:18617507). Enhances AR-mediated transactivation. Transactivation decreases as the poly-Gln length within AR increases (PubMed:10400640).
  Subcellular Location: Nucleus Nucleus envelope Cytoplasm Melanosome Predominantly nuclear during interphase. Becomes dispersed throughout the cytoplasm during mitosis. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  Unigene: Hs.10842
  SMR: P62826
  String: 9606.ENSP00000376176
  KEGG: hsa:5901
  MIM: 601179
  Pfam: PF00071
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 39.8pg/mL
• Specificity: Natural and recombinant Human GTP-binding nuclear protein Ran
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: RAN
• Gene Full Name: RAN, member RAS oncogene family
• Alias Symbols: androgen receptor-associated protein 24;ARA24;Gsp1;GTPase Ran;GTP-binding nuclear protein Ran;guanosine triphosphatase Ran;member RAS oncogene family;RanGTPase;ras-like protein TC4;ras-related nuclear protein;TC4.
• NCBI Gene Id: 5901
• Protein Name: P62826
• Description of Target: GTP-binding nuclear protein Ran
• Uniprot ID: https://www.uniprot.org/uniprot/P62826
• Protein Accession #: NP_001287725.1
• Nucleotide Accession #: NM_001300796.1