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Catalog No: OKCD00529 (Formerly GWB-KBBYG3)
Size:96WELLS
Price: $675.00
SKU
OKCD00529
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Datasheets/ManualsPrintable datasheet for RALBP1 ELISA kit (Human) (OKCD00529)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RalA Binding Protein 1 (RALBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RalA Binding Protein 1 (RALBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RalA Binding Protein 1 (RALBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RalA Binding Protein 1 (RALBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RalA Binding Protein 1 (RALBP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RalA Binding Protein 1 (RALBP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-RALBP1 Microplate96 Wells (12 x 8 Well Strips)
RALBP1 Lyophilized Standard2 x 10 ng
100X Biotinylated RALBP1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.054 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of RalA Binding Protein 1 (RALBP1). No significant cross-reactivity or interference between RalA Binding Protein 1 (RALBP1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolRALBP1
Gene Full NameralA binding protein 1
Alias Symbols76 kDa Ral-interacting protein;dinitrophenyl S-glutathione ATPase;DNP-SG ATPase;ralA-binding protein 1;ral-interacting protein 1;RIP1;RLIP1;RLIP76.
NCBI Gene Id10928
Protein NameRalA-binding protein 1
Description of TargetCan activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin.
Uniprot IDQ15311
Protein Accession #NP_006779
Nucleotide Accession #NM_006788.3
Protein Size (# AA)655
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