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RAGE ELISA Kit (Human) : 96 Wells (OKAG00245)

Catalog#: OKAG00245
Domestic: within 1 week delivery | International: 1 week
This product is Genway GWB-SKR245
More Information
Predicted Species Reactivity Human
Product Format 1 x 96-Well Plate or 12 x 8-Well Strips
Application ELISA
Kit Detection Method Colorimetric, OD450 nm
Kit Principle The Aviva Human RAGE ELISA Kit contains the components necessary for quantitative determination of natural or recombinant hRAGE concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) is bound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on the Human RAGE cytokine while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3’, 5, 5’-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.
Kit Range 63-4,000 pg/mL
Kit Component
Capture Antibody Coated Microplate96 Wells (12 x 8)
Ready-to-Use Streptavidin-HRP12 mL
Ready-to-Use Substrate12 mL
Stop Solution12 mL
Wash Buffer (15x)50 mL
Protein Standard Diluent12 mL
Sample Diluent12 mL
Detection Antibody Diluent12 mL
Biotin-Labeled Detection AntibodyLyophilized
Protein StandardLyophilized (5 ng)
Reconstitution and Storage Store as indicated in product manual.
Sample Type Cell lysates, sera and plasma
Sensitivity 63 pg/mL
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity The Human RAGE ELISA is capable of recognizing both recombinant and naturally produced Human RAGE proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Human: S100A10
The antigens listed below were tested at 6.25 ng/ml and exhibited less than 0.9% cross reactivity.
• Murine: RAGE
The antigens listed below were tested at 3.12 ng/ml and exhibited less than 8.2% cross reactivity.
• Rat: RAGE
Assay Info Quantitative Colorimentric Sandwich ELISA
Gene Symbol AGER
Official Gene Full Name advanced glycosylation end product-specific receptor
Alias Symbols Advanced glycosylation end product-specific receptor, MGC22357, RAGE, Receptor for advanced glycosylation end products
NCBI Gene Id 177
Protein Name Advanced glycosylation end product-specific receptor
Description of Target The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847).
Swissprot Id Q15109
Protein Accession # NP_001127.1
Nucleotide Accession # NM_001136.4
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express AGER.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express AGER.
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