RAGE Antibody - middle region (OAAB04025)

Catalog No: OAAB04025

Size:400ul

Western blot analysis of lysates from HepG2 cell line and human lung tissue lysates (from left to right), using RAGE Antibody (Center) (Cat. #OAAB04025). OAAB04025 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

Western blot analysis of lysate from human lung tissue lysate, using RAGE Antibody (Center)(Cat. #OAAB04025). OAAB04025 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.

Confocal immunofluorescent analysis of RAGE Antibody (Center)(Cat#OAAB04025) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IF
  Function: Able to phosphorylate several exogenous substrates and to undergo autophosphorylation.
  Cellular Location: Cytoplasm.
  Tissue Location: Expressed in heart, brain, lung, kidney, and pancreas, and at very low levels in placenta, liver and skeletal muscle. Detected in retina.
  
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This RAGE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-234 amino acids from the Central region of human RAGE.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  IF: 1:10~50

Target Info

• Gene Symbol: RAGE
• Alias Symbols: RAGE, RAGE1, STK30, RAGE-1
• NCBI Gene Id: 5891
• Protein Name: MAPK/MAK/MRK overlapping kinase
• Description of Target: RAGE is able to phosphorylate several exogenous substrates and to undergo autophosphorylation.
• Uniprot ID: Q9UQ07
• Protein Accession #: NP_055041.1
• Molecular Weight: 48014 Da