RAG2 Antibody (OAGA00787)

Catalog No: OAGA00787

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
OAGA00787 diluted at 1:1000

RAG2 antibody detects RAG2 protein by Western blot analysis.
A. 50 ug Rat thymus lysate/extract
7.5 % SDS-PAGE
RAG2 antibody (OAGA00787) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded DLD1 Xenograft, using RAG2(OAGA00787) antibody at 1:100 dilution.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RAG2(OAGA00787) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: This gene encodes a protein that is involved in the initiation of V(D)J recombination during B and T cell development. This protein forms a complex with the product of the adjacent recombination activating gene 1, and this complex can form double-strand breaks by cleaving DNA at conserved recombination signal sequences. The recombination activating gene 1 component is thought to contain most of the catalytic activity, while the N-terminal of the recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding scaffold for the tight association of the complex with DNA. A C-terminal plant homeodomain finger-like motif in this protein is necessary for interactions with chromatin components, specifically with histone H3 that is trimethylated at lysine 4. Mutations in this gene cause Omenn syndrome, a form of severe combined immunodeficiency associated with autoimmune-like symptoms. [provided by RefSeq]
  Positive Controls: Molt-4, rat thymus
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 271 and 527 of RAG2 (Uniprot ID#P55895)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.37 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: RAG2
• Gene Full Name: V(D)J recombination-activating protein 2
• Alias Symbols: RAG-2
• NCBI Gene Id: 5897
• Protein Name: V(D)J recombination-activating protein 2
• Uniprot ID: P55895
• Protein Accession #: NP_000527.2
• Molecular Weight: 59 kDa