RAD9A Antibody (OAGA00007)

Catalog No: OAGA00007

Size:100 ul

Sample (30 ug of whole cell lysate)
A: A431
B: Hela
10% SDS PAGE
OAGA00007 diluted at 1:1000

Rad9 antibody detects RAD9A protein by Western blot analysis.
A. 30 ug C2C12 whole cell lysate/extract
10 % SDS-PAGE
Rad9 antibody (OAGA00007) dilution: 1:500

Rad9 antibody detects RAD9A protein by Western blot analysis.
A. 30 ug Rat2 whole cell lysate/extract
10 % SDS-PAGE
Rad9 antibody (OAGA00007) dilution: 1:1000

Rad9 antibody detects RAD9A protein at nucleus on HBL435 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HBL435 xenograft.
Rad9 antibody (OAGA00007) dilution: 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Rad9 (OAGA00007) antibody at 1:100 dilution.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-P, WB
• Additional Information: Background: This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein is found to possess 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Use of alternative polyA sites has been noted for this gene. [provided by RefSeq]
  Positive Controls: A431, HeLa, C2C12, Rat2
  Cellular Localization: Nucleus
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment contain a sequence corresponding to a region within amino acids 197 and 391 of Rad9
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: RAD9A
• Gene Full Name: Cell cycle checkpoint control protein RAD9A
• Alias Symbols: RAD9
• NCBI Gene Id: 5883
• Protein Name: Cell cycle checkpoint control protein RAD9A
• Uniprot ID: Q99638
• Protein Accession #: NP_001230153.1
• Molecular Weight: 43 kDa