RAD9A Antibody (OAAB22020)

Catalog No: OAAB22020

Size:100ul

Immunoprecipitation analysis of Hela and 3T3 cell lysates using RAD9A Antibody (OAAB22020).

Western blot detection of RAD9A in Hela, MCF7, 3T3, COS7 and Rat testis cell lysates using RAD9A Antibody (OAAB22020) (1:500 diluted). Predicted band size: 43KDa. Observed band size: 5 5KDa.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid
• Clonality: Monoclonal
• Host: Rabbit
• Application: WB, IP
• Additional Information: Function: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. Its phosphorylation by PRKCD may be required for the formation of the 9-1-1 complex.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Predicted Homology Based on Immunogen Sequence: Human, Mouse
• Preservative: 0.02% Sodium Azide and 50% glycerol
• Formulation: Purified mouse monoclonal in PBS (pH 7.4)containing with 0.2% sodium azide,50% glycerol.
• Application Info: WB: 1:500
  IP: 1:500
• Reference: Lieberman H.B.,et al.Proc. Natl. Acad. Sci. U.S.A. 93:13890-13895(1996).
  Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
  Ota T.,et al.Nat. Genet. 36:40-45(2004).
  Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
  Roos-Mattjus P.,et al.J. Biol. Chem. 278:24428-24437(2003).
  

Target Info

• Gene Symbol: RAD9A
• Gene Full Name: RAD9 checkpoint clamp component A
• Alias Symbols: RAD9
• NCBI Gene Id: 5883
• Description of Target: This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: Q99638
• Protein Accession #: NP_001230153.1
• Nucleotide Accession #: NM_001243224.1
• Molecular Weight: 43 kDa