RAD52 Antibody (OAGA08812)

Catalog No: OAGA08812

Size:100 ul

Rad52 antibody [5H9] detects Rad52 protein by western blot analysis. Whole cell extracts (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad52 antibody [5H9] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Western blot experiment using Rad52 antibody to detect human Rad52 protein expression in HeLa whole cell lysate

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Monoclonal
• Clone: 5H9
• Isotype: IgG1
• Host: Mouse
• Application: WB
• Reconstitution and Storage: Keep as concentrated solution. Store at 4C short term. For extended storage aliquot and store at -20C or below. Avoid freeze-thaw cycles.
• Immunogen: Human Rad52 recombinant protein expressed in E. coli.
• Purification: Protein G purified
• Concentration: 2.35 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: PBS

Target Info

• Gene Symbol: RAD52
• Gene Full Name: RAD52 homolog, DNA repair protein
• NCBI Gene Id: 5893
• Protein Name: DNA repair protein RAD52 homolog
• Description of Target: The protein encoded by this gene shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. This gene product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of this gene is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
• Uniprot ID: P43351
• Protein Accession #: NP_001284348.1
• Nucleotide Accession #: NM_001297419.1