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Catalog No: OKCD01086 (Formerly GWB-KBBCT2)
Size:96WELLS
Price: $750.00
SKU
OKCD01086
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Datasheets/ManualsPrintable datasheet for RAD51 ELISA Kit (Human) (OKCD01086)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAD51 Homolog (RAD51). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAD51 Homolog (RAD51). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAD51 Homolog (RAD51), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RAD51 Homolog (RAD51) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAD51 Homolog (RAD51) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAD51 Homolog (RAD51) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-RAD51 Microplate96 Wells (12 x 8 Well Strips)
RAD51 Lyophilized Standard2 x 20 ng
100X Biotinylated RAD51 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.118 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of RAD51 Homolog (RAD51). No significant cross-reactivity or interference between RAD51 Homolog (RAD51) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolRAD51
Gene Full NameRAD51 recombinase
Alias SymbolsBRCA1/BRCA2-containing complex, subunit 5;BRCC5;DNA repair protein RAD51 homolog 1;FANCR;HRAD51;HsRad51;HsT16930;MRMV2;RAD51 homolog A;RAD51A;RECA;RecA, E. coli, homolog of;RecA-like protein;recombination protein A.
NCBI Gene Id5888
Protein NameDNA repair protein RAD51 homolog 1
Description of TargetPlays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed:28575658). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PubMed:26253028).
Uniprot IDQ06609
Protein Accession #NP_001157741
Nucleotide Accession #NM_001164269.1
Protein Size (# AA)339
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