RAD51 Antibody (Phospho-Thr309) (OASG06206)

Catalog No: OASG06206

Size:100 uL

Western Blot using HeLa-UV, 293

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: LiquidPBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IHC
• Additional Information: Cellular Localization: Nucleus Cytoplasm Cytoplasm, perinuclear region. Mitochondrion matrix Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
  Tissue Specificity: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
• Reconstitution and Storage: Store at -20°C. Avoid repeated freeze/thaw cycles.
• Immunogen: Synthesized peptide derived from human Rad51 around the phosphorylation site of T309. Location: 250-330aa.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Concentration: 1 mg/ml
• Target Post-Translational Modification: Phospho-Thr309
• Specificity: Phospho-Rad51 (T309) Polyclonal Antibody detects endogenous levels of Rad51 protein only when phosphorylated at T309.
• Application Info: IHC: 1:100~300
  ELISA: 1:5000

Target Info

• Gene Symbol: RAD51
• Gene Full Name: RAD51 recombinase
• Alias Symbols: RECA, BRCC5, FANCR, MRMV2, HRAD51, RAD51A, HsRad51, HsT16930
• NCBI Gene Id: 5888; 19361;
• Description of Target: The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: Q06609, Q08297
• Molecular Weight: 42 kDa