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RAD51 Antibody (Phospho-Thr309) (OASG06206)

100 uL
$215.00
In Stock
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Predicted Species Reactivity:
Human, Mouse
Product Format:
Liquid PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality:
Polyclonal
Isotype:
IgG
Host:
Rabbit
Application:
IHC
Additional Information:
Cellular Localization: Nucleus Cytoplasm Cytoplasm, perinuclear region. Mitochondrion matrix Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage. DNA damage induces an increase in nuclear levels. Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment. Accumulated at sites of DNA damage in a SPIDR-dependent manner.
Tissue Specificity: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
Reconstitution and Storage:
Store at -20°C. Avoid repeated freeze/thaw cycles.
Replacement Item:
This antibody may replace item sc-36360 from Santa Cruz Biotechnology.
Immunogen:
Synthesized peptide derived from human Rad51 around the phosphorylation site of T309. Location: 250-330aa.
Purification:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:
1 mg/ml
Datasheets/Manuals:
Printable datasheet for anti-RAD51 (OASG06206) antibody
Target Post-Translational Modification:
Phospho-Thr309
Specificity:
Phospho-Rad51 (T309) Polyclonal Antibody detects endogenous levels of Rad51 protein only when phosphorylated at T309.
Application Info:
IHC: 1:100~300
ELISA: 1:5000
Gene Symbol:
RAD51
Official Gene Full Name:
RAD51 recombinase
Alias Symbols:
RAD51, RAD51A, RECA, DNA repair protein RAD51 homolog 1, HsRAD51, hRAD51, RAD51 homolog A
NCBI Gene Id:
5888; 19361;
Description of Target:
The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.
Swissprot Id:
Q06609, Q08297
Molecular Weight:
42 kDa
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express RAD51.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express RAD51.

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