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Catalog No: OKCD09434
Size:96WELLS
Price: $750.00
SKU
OKCD09434
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Datasheets/ManualsPrintable datasheet for RAD23B ELISA Kit (Human) (OKCD09434)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAD23 Homolog B (RAD23B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to RAD23 Homolog B (RAD23B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain RAD23 Homolog B (RAD23B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of RAD23 Homolog B (RAD23B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RAD23 Homolog B (RAD23B) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RAD23 Homolog B (RAD23B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
RAD23B Microplate96 Wells (12 x 8 Well strips)
RAD23B Lyophilized Standard2
100X RAD23B HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.052ng/mL
Gene SymbolRAD23B
Gene Full NameRAD23 homolog B, nucleotide excision repair protein
Alias SymbolsHHR23B;HR23B;P58;RAD23, yeast homolog of, B;UV excision repair protein RAD23 homolog B;XP-C repair complementing complex 58 kDa;XP-C repair complementing protein;XP-C repair-complementing complex 58 kDa protein.
NCBI Gene Id5887
Protein NameUV excision repair protein RAD23 homolog B
Description of TargetMultiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome.
Uniprot IDP54727
Protein Accession #NP_001231653.1
Nucleotide Accession #NM_001244724.1
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