Catalog No: OCOA02506
Size:20UG
Price: $179.00
SKU
OCOA02506
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RAD18 Protein Lysate (OCOA02506)

Datasheets/ManualsPrintable datasheet for RAD18 Protein Lysate (OCOA02506)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionRAD18 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolRAD18
Gene Full NameRAD18 E3 ubiquitin protein ligase
Alias SymbolsE3 ubiquitin-protein ligase RAD18;hHR18;hRAD18;postreplication repair protein hRAD18p;postreplication repair protein RAD18;RAD18 homolog;RAD18, S. cerevisiae, homolog;RING finger protein 73;RING-type E3 ubiquitin transferase RAD18;RNF73.
NCBI Gene Id56852
Protein NameNM_020165, NP_064550, Q9NS91
Description of TargetE3 ubiquitin-protein ligase RAD18, E3 ubiquitin-protein ligase RAD18 [Homo sapiens], Homo sapiens RAD18, E3 ubiquitin protein ligase (RAD18), mRNA
Uniprot IDhttps://www.uniprot.org/uniprot/Q9NS91
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_064550
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_020165
Molecular Weight56.2 kDa
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