Catalog No: OCOA09452
Size:20UG
Price: $179.00
SKU
OCOA09452
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RAC1 Protein Lysate (OCOA09452)

Datasheets/ManualsPrintable datasheet for RAC1 Protein Lysate (OCOA09452)
Product Info
Predicted Species ReactivityHomo sapiens
Product FormatLyophilized
HostHEK293T
Additional InformationComponents: 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
::Preparation: HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reconstitution and StorageThe lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
TagC-MYC/DDK
DescriptionRAC1 HEK293T cell transient overexpression lysate (as WB positive control)
Gene SymbolRAC1
Gene Full NameRac family small GTPase 1
Alias Symbolscell migration-inducing gene 5 protein;MIG5;MRD48;p21-Rac1;Rac-1;ras-like protein TC25;ras-related C3 botulinum toxin substrate 1;ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1);TC-25.
NCBI Gene Id5879
Protein NameA4D2P0, NM_018890, NP_061485, P63000
Description of TargetHomo sapiens Rac family small GTPase 1 (RAC1), transcript variant Rac1b, mRNA, Ras-related C3 botulinum toxin substrate 1, Ras-related C3 botulinum toxin substrate 1 (Rho family, small GTP binding protein Rac1), ras-related C3 botulinum toxin substrate 1 isoform Rac1b [Homo sapiens]
Uniprot IDhttps://www.uniprot.org/uniprot/P63000
Protein Accession #https://www.ncbi.nlm.nih.gov/protein/NP_061485
Nucleotide Accession #https://www.ncbi.nlm.nih.gov/nuccore/NM_018890
Molecular Weight23.3 kDa
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