Rabies Virus Antibody (IgG) (Dog) ELISA Kit (OKCA00387)

Catalog No: OKCA00387

Size:96 Wells

Product Info

• Predicted Species Reactivity: Dog
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Principle: Aviva Systems Biology Rabies Virus Antibody (IgG) (Dog) ELISA Kit (OKCA00387) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Rabies Virus antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for DogIgG is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Rabies Virus Antibody (IgG) captured in well.
• ELISA Kit Reproducibility: Three different samples of known Rabies Virus Antibody (IgG) concentration were tested across one plate with 20 replicates and across 3 plates, 8 replicates each.
  Intra-Assay CV: <15%
  Inter-Assay CV: <15%
• ELISA Kit Component:
  • Rabies Virus Antibody (IgG) Microplate: 96 Wells (12 x 8 Well strips)
  • Negative Control: 1 x 800 uL
  • Positive Control: 1 x 800 uL
  • 100X Anti-Dog IgG HRP-Conjugate: 1 x 120 uL
  • HRP-Conjugate Diluent: 1 x 20 mL
  • Sample Diluent: 2 x 20 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum
• Assay Info: Assay Methodology: Qualitative Reverse Capture ELISA

Target Info

• Alias Symbols: RV
• Protein Name: Rabies Virus Antibody (IgG)
• Description of Target: Rabies Virus Antibody (IgG)