Donkey Anti-Rabbit IgG (H&L) Polyclonal Antibody - FITC Conjugated (OARA04849)

Catalog No: OARA04849

Size:2MG

Product Info

• Predicted Species Reactivity: Rabbit
• Product Format: Lyophilized
• Clonality: Polyclonal
• Isotype: IgG
• Host: Donkey
• Conjugation: FITC
• Application: Dot blot|Flow cytometry|Fluorescence-linked immunosorbent assay|Functional assay|Immunofluorescence|Western blot
• Additional Information: Conjugation Chemistry: Isothiocyanate
  F/P Ratio: 4.9
  Excitation: 495 nm
  Emission: 528 nm
• Reconstitution and Storage: 2°C to 8°C|-20°C or -80°C
• Immunogen: Rabbit IgG whole molecule
• Purification: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
• Concentration: 2 mg/ml
• Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
• Application Info: FLISA 1:10000~50000
  IF1:1000~5000

Target Info

• Alias Symbols: FITC
• Description of Target: Anti-Rabbit IgG (H&L) Fluorescein Antibody generated in donkey detects reactivity to Rabbit IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
• Molecular Weight: 390