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Rabbit IgG Antibody - HRP Conjugated (ASP00001)

1.6 mg
In Stock
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Product Format:
Reconstitution and Storage:
Store freeze-dried product at 2-8C until reconstitution. When ready to use, reconstitute with 2.0 ml d. water. Centrifuge product if the solution is not clear after reconstitution. To judge clarity, draw product into a clean pasteur pipette. Storing the product for more than one day at final working dilutions is not recommended. For extended storage as a liquid, add an equal volume of glycerol (ACS or better grade) for a final concentration of 50% followed by storage at -20C. Please note that the concentration of protein and buffer salts will be one-half of the original after the addition of glycerol. Alternatively, the product may be aliquoted and frozen at -70C or below, in the absence of glycerol. It is important to avoid repeated freezing and thawing. Expiration date: one year from date of reconstitution.
Suggested Dilution Range:
1:10,000 - 1:200,000 for Western blotting with ECL substrates
1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates
1:500 - 1:5,000 for immunohisto/cytochemistry
Printable datasheet for ASP00001
Based on immunoelectrophoresis, the antibody reacts with the heavy chains on rabbit IgG and with light chains common to most rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins, but the antibody may cross-react with immunoglobulins from other species.
The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Additional Information:
Antibody Concentration: 0.8 mg/ml
Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
Stabilizer: 15 mg/ml Bovine Serum Albumin
Preservative: None (Warning: Use of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.)
Note: Due to tightened regulations in many countries and states, thimerosal, a mercury-containing preservative, will no longer be included in our products. Please follow our recommendation for storage to avoid microbial contamination.

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