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Rabbit ANTI RAF-1 Antibody (OASA08611)

Catalog#: OASA08611
More Information
Product FormatPurified IgG - liquid
IsotypePolyclonal IgG
::Preservative Stabilisers: 0.09% - Sodium Azide (NaN3)
50% - Glycerol - 0.01% - Bovine Serum Albumin
Antiserum Preparation: Antisera to rat Raf-1 (pSer301) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
::Buffer Solutions: 10mMHepes pH7.5
Reconstitution and StorageStore at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
ImmunogenPhosphopeptide corresponding to amino acid sequence surrounding phosphorylated serine 301 of rat Raf-1.
Datasheets/ManualsPrintable datasheet for OASA08611
Application InfoWestern Blot: This product detects a band/s of approximately 74kDa in human jurkat cell lysates.
* For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
Target Reference1. Gollob, J. et al. (2006) Role of Raf kinase in cancer: therapeutic potential of targeting the Raf/MEK/ERK signal transduction pathway. Semin. Oncol. 4:392-406.
2. Dougherty, M. et al. (2005) Regulation of Raf-1 by direct feedback phosphorylation. Mol. Cell. 17:215-24.
Gene SymbolRaf1
Alias SymbolsRaf1
NCBI Gene Id24703
Protein NameRAF proto-oncogene serine/threonine-protein kinase
Description of TargetRABBIT ANTI RAF-1 (pSer301)
Swissprot IdP11345
Protein Accession #NP_036771.1
Protein Size (# AA)648
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express Raf1.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express Raf1.
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