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Rabbit ANTI RAF-1 Antibody (OASA08611)

  • Catalog#: OASA08611
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Gene Symbol:
Raf1
NCBI Gene Id:
24703
Protein Name:
RAF proto-oncogene serine/threonine-protein kinase
Swissprot Id:
P11345
Protein Accession #:
NP_036771.1
Alias Symbols:
Raf1
Description of Target:
RABBIT ANTI RAF-1 (pSer301)
Protein Size (# AA):
648
Host:
Rabbit
Clonality:
Polyclonal
Application:
WB
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Raf1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Raf1.
Immunogen:
Phosphopeptide corresponding to amino acid sequence surrounding phosphorylated serine 301 of rat Raf-1.
Product Format:
Purified IgG - liquid
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Datasheets/Manuals:
Printable datasheet for OASA08611
Specificity:
RAF-1
Isotype:
Polyclonal IgG
Application Info:
Western Blot: This product detects a band/s of approximately 74kDa in human jurkat cell lysates.
* For the detection of phosphoproteins, serine phosphatase inhibitors such as 10mM Sodium Fluoride should be added to the sample buffer. Milk or other casein-based blocking solutions are not recommended as casein is a phosphoprotein and its use can result in high background.
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Preservative Stabilisers: 0.09% - Sodium Azide (NaN3)
50% - Glycerol - 0.01% - Bovine Serum Albumin
Antiserum Preparation: Antisera to rat Raf-1 (pSer301) were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
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Buffer Solutions: 10mMHepes pH7.5
Target Reference:
1. Gollob, J. et al. (2006) Role of Raf kinase in cancer: therapeutic potential of targeting the Raf/MEK/ERK signal transduction pathway. Semin. Oncol. 4:392-406.
2. Dougherty, M. et al. (2005) Regulation of Raf-1 by direct feedback phosphorylation. Mol. Cell. 17:215-24.

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